| 黄小兵,朱绍兴,曾邦伟.依维莫司对前列腺癌PC-3细胞的抑制和凋亡的影响[J].肿瘤学杂志,2014,20(7):560-563. |
| 依维莫司对前列腺癌PC-3细胞的抑制和凋亡的影响 |
| Effect of Everolimus on the Growth Inhibition and Apoptosis in Prostate Cancer Cell Line PC-3 |
| 投稿时间：2013-10-29 |
| DOI：10.11735/j.issn.1671-170X.2014.07.B007 |
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| 中文关键词: 细胞凋亡 依维莫司 caspase-3 PC-3细胞 前列腺肿瘤 |
| 英文关键词:cell apoptosis everolimus caspase-3 PC-3 cells prostate neoplasms |
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| 中文摘要: |
| 摘 要：［目的］ 探讨依维莫司对前列腺癌PC-3细胞凋亡及caspase-3表达的影响。［方法］ 体外培养前列腺癌细胞PC-3，使用不同浓度依维莫司(0mol/L、10-9mol/L和10-8mol/L)干预细胞，四氮唑蓝比色法(MTT)和原位细胞凋亡检测法(TUNEL)检测依维莫司对前列腺癌细胞PC-3生长抑制和诱导细胞凋亡的作用，流式细胞术检测其对caspase-3活化的情况。［结果］ 与对照组比较，依维莫司对前列腺癌PC-3细胞抑制和诱导凋亡作用明显(P<0.05)，明显增加caspase-3的表达(P<0.05)。［结论］ 依维莫司抑制前列腺癌细胞PC-3增殖，可能通过诱导caspase-3表达活化增强，最终导致细胞凋亡。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of everolimus on the apoptosis and expression of caspase-3 in prostate cancer cell line PC-3.［Methods］ Cultures of prostate cancer cells were exposured to everolimus at concentration of 0mol/L，10-9mol/L and 10-8mol/L respectively. The effects of everolimus on growth inhibition and apoptosis was determinated with MTT and Tunnel methods. The activity of caspase-3 were determined by flow cytometry.［Results］ Compared with control group，inhibition and apoptosis of PC-3 cells by everolimus were significantly different(P<0.05)，which significantly increased the expression of caspase-3(P<0.05).［Conclusion］ Everolimus might inhibit the growth of PC-3 cells，enhance the expression of caspase-3 and lead to the apoptosis of PC-3 cells. |
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