| 宋启斌,褚玉新,胡伟国.GW843682X对鼻咽癌细胞中PLK1和p53表达的影响[J].肿瘤学杂志,2014,20(7):533-537. |
| GW843682X对鼻咽癌细胞中PLK1和p53表达的影响 |
| Effect of GW843682X on PLK1 and p53 Expression in Nasopharyngeal Carcinoma Cells |
| 投稿时间：2014-02-07 |
| DOI：10.11735/j.issn.1671-170X.2014.07.B001 |
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| 中文关键词: GW843682X 鼻咽肿瘤 Polo样激酶（PLK） p53 |
| 英文关键词:GW843682X nasopharyngeal neoplasms polo-like kinase(PLK) p53 |
| 基金项目:国家自然科学基金资助 (81372407) |
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| 摘要点击次数: 1889 |
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| 中文摘要: |
| 摘 要：［目的］ 研究Polo样激酶（PLK）抑制剂GW843682X对鼻咽癌5-8F细胞中PLK1和p53表达水平的影响。［方法］用不同浓度的GW843682X处理鼻咽癌5-8F细胞，在不同时间点倒置显微镜下观察细胞形态，RT-PCR及Western blot分别检测PLK1和p53的mRNA和蛋白表达水平的变化。［结果］ GW843682X能够显著抑制5-8F细胞的增殖，呈剂量和时间依赖性。RT-PCR结果表明GW843682X可下调PLK1和p53的mRNA表达水平。Western blot结果显示GW843682X降低PLK1和p53的蛋白表达水平。［结论］ GW843682X抑制鼻咽癌5-8F细胞中PLK1和p53的表达。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of polo-like kinase(PLK) inhibitor GW843682X on PLK1 and p53 expression in nasopharyngeal carcinoma cell lines 5-8F.［Methods］ Nasopharyngeal carcinoma 5-8F cells were treated with different concentrations of GW843682X. At different time points，celluar morphology was observed under inverted microscope. RT-PCR and Western blot were used to detect mRNA and protein expression of PLK1 and p53. ［Results］ GW843682X remarkably inhibited the proliferation of 5-8F cells in a dose-dependent and time-dependent manner with obvious morphologic changes. The results of RT-PCR indicated that GW843682X down-regulated the mRNA level of PLK1 and p53. The results of Western blot showed that GW843682X suppressed the protein level of PLK1 and p53. ［Conclusion］ GW843682X might inhibit PLK1 and p53 expression in 5-8F cell lines. |
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