| 李鹏熙,李 毅,李东红.新型靶向光敏剂光动力诱导HeLa细胞凋亡的实验研究[J].肿瘤学杂志,2014,20(5):383-387. |
| 新型靶向光敏剂光动力诱导HeLa细胞凋亡的实验研究 |
| An Experimental Study on Apoptosis of HeLa Cells Induced by A New Targeting Photosensitizer-based Photodynamic Therapy (PDT) |
| 投稿时间：2013-12-12 |
| DOI：10.11735/j.issn.1671-170X.2014.05.B007 |
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| 中文关键词: 靶向性光敏剂Ⅰ HeLa细胞 光动力治疗 细胞凋亡 |
| 英文关键词:targeting photosensitizer Ⅰ HeLa cells photodynamic therapy cell apoptosis |
| 基金项目:国家自然科学基金资助项目（21072227） |
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| 摘要点击次数: 2060 |
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| 中文摘要: |
| 摘 要：［目的］ 研究新型靶向光敏剂Ⅰ光动力诱导HeLa细胞死亡的方式。［方法］ 用流式细胞仪分析照射剂量和光敏剂浓度对HeLa细胞凋亡的影响，细胞凋亡和坏死的比例；并采用激光共聚焦观察细胞F-actin和核的变化；透射电镜观察细胞超微结构的改变。［结果］ 光敏剂Ⅰ介导的光动力治疗可诱导HeLa细胞的凋亡和坏死，但凋亡的比例明显大于坏死(P=0.0053)，且细胞凋亡的量分别与光照剂量（r=0.987，P<0.001）和光敏剂浓度（r=0.995，P<0.001）呈正相关；光动力诱导后，细胞的F-actin 结构破坏，细胞皱缩，透射电镜下可见凋亡小体的形成。［结论］ 光敏剂Ⅰ介导的光动力治疗主要通过凋亡途径导致HeLa细胞的死亡。 |
| 英文摘要: |
| Abstract：［Purpose］ To study the death mode of HeLa cells induced by a new targeting photosensitizer Ⅰ-based photodynamic therapy (PDT). ［Methods］ The influence of irradiation dose and the concentration of photosensitizer Ⅰ on the apoptosis of HeLa cells，and the percentage of apoptosis and necrosis of HeLa cells were analyzed by flow cytometry. The changes of F-actin and nuclear of HeLa cells were observed through confocal. The changes of ultrastructure were observed under transmission electron microscope. ［Results］ Compared with control cells，photosensitizer Ⅰ-based PDT could induce apoptosis and necrosis in HeLa cells，but the percentage of apoptotic cells was obviously more than that of necrosis，and the number of apoptotic cells displayed positive correlation with the irradiation dose and the concentration of photosensitizer Ⅰ，the correlation coefficients were 0.987(P<0.001) and 0.995(P<0.001) respectively. After PDT，the F-actin of HeLa cells was damaged，the nucleus shrank，and the apoptotic bodies could be observed under transmission electron microscope.［Conclusion］ Apoptosis was the main death mode of HeLa cells induced by photosensitizer Ⅰ-based PDT. |
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