| 应吟吟,徐伟珍,毛伟敏.大蒜提取物S-烯丙基半胱氨酸对人食管鳞癌TE-13细胞株增殖和凋亡作用的研究[J].肿瘤学杂志,2014,20(4):294-299. |
| 大蒜提取物S-烯丙基半胱氨酸对人食管鳞癌TE-13细胞株增殖和凋亡作用的研究 |
| Effect of Garlic Derivative S-allylcysteine (SAC) on Proliferation and Apoptosis of Esophageal Squamous Cell Carcinoma TE-13 Cell Line |
| 投稿时间：2014-02-23 |
| DOI：10.11735/j.issn.1671-170X.2014.04.B007 |
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| 中文关键词: 食管肿瘤 S-烯丙基半胱氨酸 大蒜 抗肿瘤药物 STAT3 增殖 凋亡 |
| 英文关键词:esophageal neoplasms S-allylcysteine garlic antitumor drug STAT3 proliferation apoptosis |
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| 中文摘要: |
| 摘 要：［目的］ 研究S-烯丙基半胱氨酸(SAC)对人食管鳞癌细胞株TE-13增殖、凋亡等生物学特性的影响。［方法］ MTT比色法和细胞克隆形成实验检测SAC作用于人食管鳞癌细胞株TE-13的增殖活性，流式细胞术分析肿瘤细胞的凋亡，Western Blot检测细胞内STAT3、Mcl-1、Survivin蛋白的表达。［结果］ SAC明显抑制TE-13细胞的增殖率和克隆形成率，并且具有时间和浓度依赖性。进一步研究发现SAC作用于细胞后通过抑制STAT3、Mcl-1和Survivin蛋白的表达，活化Active-caspase 3蛋白的表达从而诱导细胞凋亡和坏死。［结论］ S-烯丙基半胱氨酸通过抑制STAT3、Mcl-1和Survivin的表达，进而抑制TE-13细胞的增殖，诱导其凋亡。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of S-allylcysteine (SAC) on proliferation and apoptosis of human esophageal squamous cell carcinoma TE-13 cell line.［Methods］ Cell prolifera-tion was detected by MTT and colony-forming assays. Cell apoptosis was detected by Annexin Ⅴ/PI staining with flow cytometry. The expression of STAT3，Mcl-1 and Survivin was detected by Western Blot assay.［Results］ The proliferation rate and colony-forming abilities of TE-13 cells were suppressed by SAC in dose-dependent and time-dependent manners. Moreover，SAC significantly induced apoptosis and necrosis of TE-13 cells through suppressing STAT3，Mcl-1，Survivin as well as activating caspase 3.［Conclusion］ SAC inhibits cell proliferation and induces apoptosis by inhibiting STAT3 signal transduction in human esophageal squamous cell carcinomas TE-13 cell line. |
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