| 汤海标,蔡锦威,刘卫国.丹皮酚对乳腺癌细胞的生长抑制作用[J].肿瘤学杂志,2014,20(4):276-281. |
| 丹皮酚对乳腺癌细胞的生长抑制作用 |
| Effects of Paeonol on Growth Inhibition of Human Breast Cancer Cells |
| 投稿时间：2014-02-25 |
| DOI：10.11735/j.issn.1671-170X.2014.04.B003 |
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| 中文关键词: 丹皮酚 乳腺肿瘤 细胞增殖 凋亡 |
| 英文关键词:Paeonol breast neoplasms cell proliferation apoptosis |
| 基金项目:浙江省自然科学基金（Y2101312）；浙江省医药卫生科技计划（2010QNA006） |
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| 中文摘要: |
| 摘 要：［目的］研究丹皮酚(Pae)对人乳腺癌细胞的作用及机制。［方法］采用MTT法检测Pae对乳腺癌Bcap37、MDA-MB-453细胞的增殖抑制作用，流式细胞仪检测Pae 对Bcap37和MDA-MB-453细胞凋亡和周期分布的影响，Western Blot检测相关蛋白表达。［结果］Pae对乳腺癌Bcap37及MDA-MB-453细胞均具有增殖抑制作用，并呈时间和剂量依赖性；流式细胞仪分析细胞凋亡率呈浓度依赖性，细胞周期分析显示Pae 阻滞Bcap37及MDA-MB-453细胞在G1期；Western Blot检测凋亡相关蛋白 Caspase 7、Caspase 3和PARP表达减少，而PTEN蛋白表达增加。［结论］Pae 对乳腺癌细胞系具有增殖抑制作用，其机制可能与促进细胞凋亡及引起细胞周期阻滞，PTEN表达上调有关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of Pae on proliferation of human breast cancer cells and its possible influencing mechanism．［Methods］ MTT assay was used to measure the effect of Pae on proliferation of breast cancer cells Bcap37 and MDA-MB-453. Apoptosis and cell cycle were measured by flow cytometry. Western Blot was used for detection of protein expression．［Results］ Pae showed inhibitory effect on Bcap37 and MDA-MB-453 breast cancer cells in time- and dose-dependent．Bcap-37 and MDA-MB-453 cells treated by Pae were blocked in G1 phase. The apoptosis rate appeared in dose-dependent．Western Blot showed that the expressions of Caspase 3，Caspase 7 and PARP decreased and the expression of PTEN increased．［Conclusion］ Pae can inhibit the growth of breast cancer cells，which is possibly contribute to apoptosis inducing and cell cycle arrest． |
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