| 张 博,张雪鹏,胡宝山.缺氧对人乳腺癌MCF-7细胞miRNA-21表达的影响及与细胞增殖和凋亡的关系[J].肿瘤学杂志,2014,20(4):265-269. |
| 缺氧对人乳腺癌MCF-7细胞miRNA-21表达的影响及与细胞增殖和凋亡的关系 |
| Influence of Hypoxia on the Expression of miRNA-21 in Human Breast Cancer MCF-7 Cell and Its Relationship with Cell Proliferation and Apoptosis in Vitro |
| 投稿时间：2013-12-04 |
| DOI：10.11735/j.issn.1671-170X.2014.04.B001 |
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| 中文关键词: 乳腺肿瘤 缺氧微环境 微小RNA-21 缺氧诱导因子-1α |
| 英文关键词:breast neoplasms hypoxia microenvironment microRNA-21 hypoxia inducible factor-1α (HIF-1α) |
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| 摘要点击次数: 2019 |
| 全文下载次数: 1257 |
| 中文摘要: |
| 摘 要：［目的］ 观察缺氧环境下人乳腺癌MCF-7细胞增殖及凋亡的情况，初步探讨缺氧对miRNA-21表达的影响及其与细胞增殖、凋亡的关系。［方法］ CoCl2人工模拟缺氧环境，四甲基偶氮唑蓝比色法（MTT）检测缺氧环境下细胞增殖状况，流式细胞仪检测细胞的凋亡率，实时荧光聚合酶链反应（RT-PCR）测定不同缺氧程度下HIF-1α和miRNA-21表达水平的变化。［结果］ 与常氧对照组相比较，CoCl2处理组的细胞明显受到抑制，但100、200μmol/L CoCl2处理组的细胞在一定时间内仍增殖，抑制率随缺氧时间和药物浓度增加而上升。流式细胞仪检测结果显示，CoCl2处理组细胞凋亡率高于对照组（P＜0.05）。RT-PCR检测显示，200、400μmol/L的CoCl2处理组培养24h后细胞的HIF-1α和miRNA-21表达高于对照组（P＜0.05）。［结论］ 人乳腺癌MCF-7细胞可在一定缺氧程度和时间内生长，缺氧诱导细胞凋亡率增加，缺氧能上调HIF-1α、miRNA-21的表达，使之进一步耐受缺氧，其机制可能是上调的miRNA-21通过多个信号通路调控靶基因进而影响癌细胞的增殖及凋亡。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effects of hypoxia microenvironment on the proliferation and apoptosis of human breast cancer MCF-7 cell line，and preliminarily explore the effects of hypoxia on the cellular expression of microRNA-21(miRNA-21) and the cell proliferation and apoptosis. ［Methods］ Hypoxia environment of breast cancer line MCF-7 was induced by CoCl2. MTT assay was used to monitor the situation of CoCl2-induced hypoxia on proliferation of human breast MCF-7 cell in vitro. The cell apoptotic ratio was detected by flow cytometry. Reverse transcription polymerase chain reaction (RT-PCR) was used to examine the expression of HIF-1α mRNA in different hypoxia level，and the expression of miRNA-21 was assessed by stem-loop RT-PCR. ［Results］ Compared with the cells without CoCl2 treatment，the cells with CoCl2 exposure exhibited obvious growth inhibition which increased with CoCl2 concentration and exposure time，but 100，200μmol/L CoCl2 treatment group still had proliferation of cells in a certain period of time. According to the flow cytometry results，apoptosis rate in CoCl2 treatment group was higher than that in control group. The mRNA expressions of HIF-1α and miRNA-21 were higher in 200，400μmol/L CoCl2 treatment group than those in control group(P<0.05). ［Conclusions］ Human breast cancer MCF-7 cell are grown in a certain degree of hypoxia and time. The increase of cell apoptosis is induced by hypoxia. Hypoxia condition can up-regulate the expression of HIF-1α and miRNA-21，lead to hypoxia tolerance of MCF-7. The mechanism may be miRNA-21 up-regulated through multiple signaling pathways regulating target genes to affect cancer cell proliferation and apoptosis. |
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