| 江 皓,赵鹏军,马胜林.重楼皂苷Ⅰ通过PI3K/Akt途径诱导胰腺癌PANC-1细胞凋亡的研究[J].肿瘤学杂志,2014,20(2):127-130. |
| 重楼皂苷Ⅰ通过PI3K/Akt途径诱导胰腺癌PANC-1细胞凋亡的研究 |
| The Effect of Paris Saponin Ⅰ on Apoptosis Associating with PI3K/Akt Pathway in Pancreatic Carcinoma Cell Line PANC-1 |
| 投稿时间：2013-08-14 |
| DOI：10.11735/j.issn.1671-170X.2014.02.B010 |
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| 中文关键词: 胰腺肿瘤 重楼皂甙Ⅰ 细胞凋亡 |
| 英文关键词:pancreatic neoplasms Paris Saponin Ⅰ apoptosis |
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| 摘要点击次数: 1976 |
| 全文下载次数: 840 |
| 中文摘要: |
| 摘 要：［目的］ 评价重楼皂甙Ⅰ对胰腺癌PANC-1细胞增殖和凋亡的影响。［方法］ 以体外培养的胰腺癌PANC-1细胞为研究对象，MTT 法检测重楼皂甙Ⅰ对PANC-1细胞增殖的抑制作用，Annexin-Ⅴ-FITC/PI 双染法检测细胞凋亡，Western Blot 法检测PI3K、Akt、pAkt、Bax、Bcl-2、caspase-3蛋白表达改变。［结果］ 不同浓度重楼皂甙Ⅰ能有效抑制PANC-1细胞的增殖，且呈时间浓度依赖性（P＜0.01）。低、中、高浓度重楼皂甙Ⅰ作用PANC-1细胞24、48h 后，细胞凋亡率明显增加，与对照组相比，具有统计学差异（P＜0.01）。2μg/ml 重楼皂甙Ⅰ作用PANC-1细胞48h 后，PI3K、pAkt、Bcl-2蛋白表达降低，caspase-3、Bax蛋白表达增加，与对照组相比，具有统计学差异（P＜0.01）。［结论］ 重楼皂甙Ⅰ能抑制胰腺癌PANC-1细胞的体外增殖，诱导细胞凋亡，机制可能与降低PI3K、pAkt、Bcl-2 蛋白表达，增加Bax 及caspase-3 蛋白表达有关。 |
| 英文摘要: |
| Abstract:［Purpose］ To investigate the effect of Paris Saponin Ⅰ on proliferation and apoptosis in pancreatic carcinoma cell line PANC-1.［Methods］ The effects of Paris Saponin Ⅰ on cell proliferation and cell apoptosis were detected by MTT method and Annexin-Ⅴ-FITC/PI respectively. The expressions of PI3K，Akt，pAkt，Bax，Bcl-2 and caspase-3 were detected by Western Blot.［Results］ The growth of PANC-1 cells was inhibited by Paris Saponin Ⅰ in dose-dependent and time-dependent manner. Cell apoptosis was induced significantly after treated with different concentration of Paris Saponin Ⅰ at 24h and 48h(P<0.01). The expression of PI3K，pAkt and Bcl-2 protein decreased，while the expressions of Bax and caspase-3 protein increased after treated with 2μg/ml Paris SaponinⅠ at 48h.［Conclusion］ Paris Saponin Ⅰ can inhibit cell proliferation of pancreatic carcinoma cell line PANC-1 in dose-dependent and time-dependent manner and induce cell apoptosis，associating with decreasing expression of PI3K，pAkt and Bcl-2，increasing expression of Bax and caspase-3. |
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