蒋传命,黄泽智,杨 秦.人参皂苷Compound K对慢性粒细胞白血病K562细胞系凋亡诱导作用及其机制的研究[J].肿瘤学杂志,2014,20(2):122-126. |
人参皂苷Compound K对慢性粒细胞白血病K562细胞系凋亡诱导作用及其机制的研究 |
Effect of Apoptosis Inducing of Ginsenoside Compound K on Chronic Myelocytic Leukemia K562 Cells and Its Mechanism |
投稿时间:2013-07-26 |
DOI:10.11735/j.issn.1671-170X.2014.02.B009 |
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中文关键词: 人参皂苷CK K562细胞 细胞凋亡 白血病 |
英文关键词:Ginsenoside CK K562 cells apoptosis leukemia |
基金项目:湖南省教育厅科学研究项目基金(11C1159) |
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中文摘要: |
摘 要:[目的] 探讨人参皂苷Compound K(CK)对慢性粒细胞白血病K562细胞凋亡的诱导作用及其机制。[方法]应用MTT法检测CK对K562细胞增殖的影响,用流式细胞术分析细胞凋亡情况,半定量RT-PCR检测Caspase3、Caspase9、Bcl-2和Bax的mRNA表达水平,Western Blot检测Caspase3、Caspase9、Bcl-2和Bax蛋白质的表达。[结果] 人参皂苷CK能诱导K562细胞的凋亡,细胞凋亡率呈浓度依赖性增加。RT-PCR结果显示Caspase3、Caspase9 mRNA的表达上调,Bcl-2 mRNA的表达下调,Western Blot实验显示蛋白质表达情况与RT-PCR结果一致,Caspase3、Caspase9蛋白质的表达上调,Bcl-2的表达下调。[结论] 人参皂苷CK诱导K562细胞凋亡作用的机制可能是CK抑制Bcl-2基因的表达,进而使Caspase3、Caspase9表达量上调,启动凋亡途径,引起凋亡。 |
英文摘要: |
Abstract:[Purpose] To investigate the apoptosis of Ginsenoside CK on the K562 cells and its mechanism. [Methods] The proliferation inhibition effects of Ginsenoside CK on the K562 cells were determined by MTT assay. K562 cells apoptotic percentage was detected by flow cytometry. The mRNA expression of Caspase3,Caspase9,Bcl-2 and Bax was detected by semi-quantitative RT-PCR. Western Blot assay was carried out to examine Caspase3,Caspase9,Bcl-2 and Bax protein expression. [Results] Ginsenoside CK significantly inhibited the proliferation of K562 cells in dose-dependent and time-dependent manners. In addition,apoptosis percentage of K562 cells can be induced by Ginsenoside CK in dose-dependent manner. The expression of Caspase3,Caspase9 mRNA was obviously up-regulated,while the Bcl-2 expression was down-regulated. The result of Western Blot assay was accord with that of RT-PCR. The expression of Caspase3,Caspase9 protein was obviously up-regulated,while the Bcl-2 expression was down-regulated.[Conclusion] Ginsenoside CK plays a crucially important role in exerting its anti-leukemic effects via induction of apoptosis in K562 cells. This effect is possibly to inhibit Bcl-2 expression,which may occur apoptosis through decreasing Bcl-2 expression down-regulated by caspase pathway. |
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