| 李 荣,吴裕中,吴 强.Vasohibin-1重组腺病毒对人卵巢癌SKOV-3细胞增殖的影响[J].肿瘤学杂志,2014,20(1):1-5. |
| Vasohibin-1重组腺病毒对人卵巢癌SKOV-3细胞增殖的影响 |
| Effects of Vasohibin-1 Recombinant Adenovirus on Proliferation of Human Ovarian Cancer SKOV-3 Cells |
| 投稿时间：2013-09-07 |
| DOI：10.11735/j.issn.1671-170X.2014.01.B001 |
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| 中文关键词: 卵巢肿瘤 SKOV-3细胞 vasohibin-1 腺病毒 细胞增殖 |
| 英文关键词:ovarian neoplasms SKOV-3 cells vasohibin-1 adenovirus cell proliferation |
| 基金项目:江苏省科技厅社会发展项目资助课题（BS2006072，BS2002058）；江苏省中医药局课题（LZ13234，H07029）；江苏省肿瘤医院课题（ZS201202，ZM201103） |
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| 中文摘要: |
| 摘 要：［目的］研究内源性血管生成抑制因子vasobihin-1对人卵巢癌SKOV-3细胞体外增殖能力的影响。［方法］ 体外培养SKOV-3细胞，用vasohibin-1重组腺病毒感染SKOV-3细胞，采用蛋白质印迹法检测SKOV-3细胞中vasohibin-1蛋白的表达水平，应用CCK-8法检测不同时间点vasohibin-1重组腺病毒对SKOV-3细胞增殖的抑制能力。［结果］ 感染48h后，在SKOV-3细胞中vasohibin-1蛋白表达量与对照组相比显著性增加（P<0.05）；25MOI vasohibin-1重组腺病毒感染SKOV-3细胞24h时，过表达组与阴性对照相比，两者之间没有统计学差异（P=0.065）；余各组间相比差异均具有统计学意义（P<0.05），且抑制能力随MOI增大而增加（P<0.05），随时间的延长而增加（P<0.05）。［结论］ Vasohibin-1过表达能够抑制SKOV-3细胞的增殖能力，并具有浓度和时间依赖性，可为卵巢癌的治疗提供新思路。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the effects of the endogenous angiogenesis inhibitor vasohibin-1 on proliferation of the human ovarian cancer SKOV-3 cells.［Methods］ SKOV-3 cells were cultured in vitro，then transfected by vasohibin-1 recombinant adenovirus. The expression of vasohibin-1 protein in SKOV-3 cells was confirmed by Western blot. The inhibition of proliferation in SKOV-3 cells were estimated by CCK8 in different times.［Results］Compared with control group，the expression of vasohibin-1 protein increased significantly after transfection for 48h(P<0.05).There was no statistical difference between overexpression group and negative control group after 25MOI vasohibin-1 recombinant adenovirus transfected SKOV-3 cells for 24h(P=0.065)；but the other groups had statistical significance and the inhibition of vasohibin-1 recombinant adenovirus on the proliferation of SKOV-3 cells increased with the growth of MOI and with the time prolonging(P<0.05). ［Conclusion］ Overexpression of vasohibin-1 could inhibit the proliferation of SKOV-3 cells，with dose- and time- dependent manners，which may provide a new idea for ovarian cancer treatment. |
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