| 谢勋鹏,冉立峰,范艳敏.HIFU活化的CTL免疫治疗后荷瘤鼠免疫功能变化[J].肿瘤学杂志,2013,19(9):694-698. |
| HIFU活化的CTL免疫治疗后荷瘤鼠免疫功能变化 |
| Immunity Changes After Immunotherapy by HIFU Activated CTL in the Homogeneous Tumor Bearing Mice |
| 投稿时间：2013-06-03 |
| DOI：10.11735/j.issn.1671-170X.2013.09.B007 |
|
 |
| 中文关键词: 高强度聚焦超声 肿瘤 免疫治疗，过继 T淋巴细胞，杀伤性 |
| 英文关键词:high intensity focused ultrasound (HIFU) neoplasms immunotherapy,adoptive T lymphocytes,cytotoxic |
| 基金项目: |
|
| 摘要点击次数: 2812 |
| 全文下载次数: 1239 |
| 中文摘要: |
| 摘 要：［目的］ 探讨过继免疫高强度聚焦超声( HIFU) 治疗后活化的杀伤性T淋巴细胞（CTL）引起的荷瘤鼠肿瘤免疫功能变化。［方法］ C57BL/ 6J 近交系荷瘤鼠在H22肝癌移植后7d分别接受HIFU治疗和HIFU假照治疗，治疗后14d分离两组荷瘤鼠和正常小鼠外周血CTL。选30只H22荷瘤鼠随机分为治疗组、假照组和对照组，分别尾静脉注射之前分离的3组CTL。于过继免疫治疗后2周测定各组小鼠外周血T淋巴细胞亚群的变化，采用MTT法测定脾淋巴细胞的体外杀伤活性，ELISA法检测脾淋巴细胞与肿瘤细胞共培养24h后上清液IFN-γ、TNF-α含量。［结果］ CTL过继免疫治疗后2周，与假照组和对照组比较，治疗组小鼠肿瘤体积较小，外周血CD4+水平明显增加，CD8+ 水平明显降低，CD4+/CD8+ 的比值明显升高，脾脏淋巴细胞对H22肿瘤细胞毒活性明显增加，与H22肿瘤细胞共培养上清液INF-γ和TNF-α浓度明显增加，差异有显著统计学意义。［结论］ HIFU活化的CTL过继免疫治疗H22 移植性肝癌，宿主细胞免疫功能增强，同时机体产生抗肿瘤的免疫作用。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the immunity changes induced by high intensity focused ultrasound (HIFU) activated cytotoxic T lymphocytes（CTLs） in the homogeneous tumor bearing mice. ［Methods］ C57BL/6J mice underwent HIFU ablation and sham-HIFU ablation 7 days after tumor implantation. Fourteen days after ablation，peripheral blood CTLs were extracted and purified in each treated mouse and normal mouse. Thirty C57BL/ 6J mice bearing H22 tumor were randomly divided into HIFU group (n=10)，sham-HIFU group (n=10)，and control group (n=10). Through caudal vein injection each mouse in 3 groups received adoptive immunotherapy with peripheral blood CTLs. Two weeks after the adoptive immunotherapy with HIFU-activated CTLs，peripheral blood was collected in each mouse to detect T lymphocyte and subset. Splenic lymphocytes were also extracted to assess the cytotoxic activity against H22 tumor cells by MTT assay. Furthermore，both IFN-γ and TNF-α levels were respectively detected in the supernatant of culture by ELISA after a 24-hour culture of spleen lymphocytes with H22 cells. ［Results］ Two weeks after adoptive immunotherapy，compared to the values in either sham-HIFU group or control group，there were a significant decrease of tumor volume and CD8+ level，and a significant increase of both CD4+ level and CD4+/CD8+ ratio in HIFU group. A significant increase of splenic lymphocyte cytotoxicity on H22 tumor cells in vitro was observed in HIFU group. Compared to the values in either sham-HIFU group or control group，there was a significant increase of both IFN-γ and TNF-α levels in HIFU group. ［Conclusion］ The immunity is improved in the blood after adoptive immunotherapy with HIFU-activated CTLs for implanted homogeneous tumor in the host，and the anti-tumor immune activities are improved． |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |