| 苏 芳,张玉霞,陈盼盼.血液病患者人巨细胞病毒感染的实验室诊断[J].肿瘤学杂志,2013,19(8):632-635. |
| 血液病患者人巨细胞病毒感染的实验室诊断 |
| Diagnosis of Human Cytomegalovirus Infection in Patients with Hematological Malignancies |
| 投稿时间：2013-06-18 |
| DOI：10.11735/j.issn.1671-170X.2013.08.B010 |
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| 中文关键词: 人巨细胞病毒 抗体 HCMV-DNA HCMV-pp65 |
| 英文关键词:human cytomegalovirus antibody HCMV-DNA HCMV-pp65 |
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| 中文摘要: |
| 摘 要:［目的］ 探讨人巨细胞病毒（HCMV）在血液病患者中的感染状况。［方法］ 共收集到血液病患者136例，应用酶联免疫吸附实验(ELISA)测定HCMV-IgG和HCMV-IgM，应用实时荧光定量—多聚酶链反应(real-time PCR)和细胞免疫组化染色法检测外周血白细胞中HCMV-DNA和HCMV-pp65抗原。［结果］ 血液病患者HCMV-IgG、HCMV-IgM、HCMV-DNA和HCMV-pp65阳性检出率分别为99.3%、2.2%、18.8%和12.5%。而健康对照组除HCMV-IgG阳性率为100%外，其余均为阴性。［结论］ 血液病患者存在不同程度的HCMV感染。临床上开展HCMV抗原、抗体及核酸的检测，对血液病患者早期防治HCMV感染具有重要意义。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate human cytomegalovirus (HCMV) infection in patients with malignant hematologic diseases.［Methods］ Anti-HCMV antibody levels were determined by enzyme-linked immunosorbent assay (ELISA)，HCMV-DNA by polymerase chain reaction (PCR) at the same time in 136 patients suffering from malignant hematologic diseases. HCMV-pp65 antigen was determined by immunohistochemistry staining (IHC). ［Results］ The positive rates of the HCMV-IgG，HCMV-IgM，HCMV-DNA and pp65 were 99.3%，2.2%，18.8% and 12.5% respectively.［Conclusions］ HCMV infects patients with malignant hematologic diseases in varying degrees. Monitoring the pp65 antigen and anti-HCMV antibodies as well as HCMV-DNA plays important roles in the early prevention and treatment for malignant hematologic patients. |
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