| 罗丽丽,陈淑慧,熊 艳.HIV阳性女性患者P16INK4a蛋白表达与HPV感染相关性分析[J].肿瘤学杂志,2013,19(8):600-604. |
| HIV阳性女性患者P16INK4a蛋白表达与HPV感染相关性分析 |
| The Relationship Between Expression of P16INK4a Protein and HPV Infection in HIV-positive Women |
| 投稿时间：2013-02-05 |
| DOI：10.11735/j.issn.1671-170X.2013.08.B004 |
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| 中文关键词: 人类免疫缺陷病毒 人乳头瘤病毒 P16INK4a蛋白 |
| 英文关键词:human immunodeficiency virus human papilloma virus P16INK4a protein |
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| 中文摘要: |
| 摘 要：［目的］了解HIV阳性感染者宫颈脱落细胞P16INK4a蛋白表达与HPV感染的相关性。［方法］ 采用通用引物PCR和反向点杂交技术建立的检测方法，对182例HIV阳性感染者和300例HIV阴性感染者检测23种HPV亚型，包括18种高危型和5种低危型。同时采用免疫组织化学技术（S-P染色法）检测宫颈脱落细胞中P16INK4a蛋白表达情况。［结果］ HIV阳性女性HPV感染率（36.8%）及P16INK4a蛋白阳性表达率（18.7%）较HIV阴性者（19.3%、4.3%）高（P均为0.000）。HIV阳性患者中，HPV感染阳性者宫颈脱落细胞P16INK4a蛋白表达率为43.3%，HPV阴性者4.3%(P=0.000)；HPV多型感染者宫颈脱落细胞P16INK4a蛋白表达率（60.9%）较HPV单型感染者高（34.1%）（P=0.042）；高危型HPV感染者宫颈脱落细胞P16INK4a蛋白表达率（80.6%）较低危型HPV感染者高（0）（P=0.000）。HIV阴性患者中，HPV感染阳性者P16INK4a蛋白阳性表达率（5.2%）较HPV阴性者（1.3%）高（P=0.721）；所检HPV阴性患者均为单一HPV感染；高危型HPV感染患者P16INK4a蛋白阳性表达率（40.0%）较低危型（1.9%）明显增高（P=0.018）。［结论］ HIV阳性女性中HPV感染阳性、多型HPV感染及高危型HPV感染宫颈细胞P16INK4a蛋白的表达率更高。 |
| 英文摘要: |
| Abstract:［Purpose］ To investigate the relationship between the P16INK4a protein expression in cervical exfoliated cells of HIV-positive women and the HPV infection.［Methods］ Twenty-three HPV subtypes，including 18 high-risk types and 5 low-risk types were detected by the methods of universal primer PCR and reverse dot blot technique in 182 HIV-positive women and 300 HIV-negative women. The P16INK4a protein of the cervical exfoliated cells was detected by immunohistochemistry (S-P staining). ［Results］ In HIV-positive women，the infection rate of HPV(36.8%) and P16INK4a protein expression (18.7%) were higher than those in HIV-negative women(19.3%，4.3%)(P=0.000). In HIV-positive women，the P16INK4a protein expression of the cervical exfoliated cells with HPV infection was significantly higher (43.3%) than that in the women with negative HPV infection(4.3%)(P=0.000). In the women with multiple HPV infection，the P16INK4a protein expression was higher (60.9%) than that in women with single HPV infection(34.1%)（P=0.042）. In women with high-risk HPV infection，the P16INK4a protein expression was higher (80.6%) than that in women with low-risk HPV infection(0)(P=0.000). In HIV-negative group，the expression of P16INK4a protein was higher than that in the HPV-infected women（5.2%）（P=0.721）. All of the HIV-negative women were single infected by HPV. In the high-risk HPV infection（40.0%），the P16INK4a protein expression was higher than that in the low-risk infected women（1.9%）（P=0.018）. ［Conclusion］ In cervical exfoliated cells of HIV-positive women，the P16INK4a protein expression in the group with positive HPV infection，multiple HPV infection and high-risk HPV infection are significantly higher. |
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