鲁 露,李争光,吴昌平.鱼藤素和氟尿嘧啶对胃癌细胞株MGC-803的协同抗肿瘤作用[J].肿瘤学杂志,2013,19(7):523-526.
鱼藤素和氟尿嘧啶对胃癌细胞株MGC-803的协同抗肿瘤作用
Synergistic Antitumor Effect of Deguelin Combined with 5-Fluorouracil on Gastric Carcinoma Cell Line MGC-803
投稿时间:2013-01-16  
DOI:10.11735/j.issn.1671-170X.2013.07.B005
中文关键词:  鱼藤素  氟尿嘧啶  胃肿瘤  协同作用
英文关键词:deguelin  5-Fluorouracil  gastric neoplasms  chemosensitization
基金项目:
作者单位
鲁 露 苏州大学附属第三医院 
李争光 苏州大学附属第三医院 
吴昌平 苏州大学附属第三医院 
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中文摘要:
      摘 要:[目的] 观察鱼藤素与氟尿嘧啶联用对胃癌细胞的作用。[方法] 胃癌MGC-803细胞经鱼藤素、氟尿嘧啶单药及两药联合处理48h后,MTT法检测其对细胞生长增殖抑制水平,采用等效图解法分析其作用;流式细胞术检测经鱼藤素处理后MGC-803细胞周期的变化。[结果] 鱼藤素可剂量依赖性抑制MGC-803细胞增殖,有G1期阻滞作用,与氟尿嘧啶联用可明显增强细胞杀伤作用。[结论] 鱼藤素与氟尿嘧啶联用可产生明显协同作用,机制为两者作用于不同细胞周期,增强了细胞对氟尿嘧啶的敏感性。
英文摘要:
      Abstract: [Purpose] To investigate the effects of deguelin combined with 5-Fluorouracil on gastric carcinoma cells. [Methods] Human gastric carcinoma cell line MGC-803 was used in present study. The inhibition effect on cell proliferation was determined by the MTT assay 48h after treated with deguelin,5-Fluorouracil and the combination. The results of MTT assays were analyzed by isobologram. Cell cycle distribution was determined by flow cytometry after treated with deguelin. [Results] Deguelin inhibited proliferation of MGC-803 cells in dose-dependent manner. After treated with deguelin,the cell cycle was arrested in G1 phase. The killing effect of 5-Fluorouracil to MGC-803 cells markedly increased after combination with deguelin. [Conclusion] Combination of deguelin and 5-Fluorouracil exhibits synergistic effects on MGC-803 cells because the two drugs act on different cell cycle phase.
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