| 鲁 露,李争光,吴昌平.鱼藤素和氟尿嘧啶对胃癌细胞株MGC-803的协同抗肿瘤作用[J].肿瘤学杂志,2013,19(7):523-526. |
| 鱼藤素和氟尿嘧啶对胃癌细胞株MGC-803的协同抗肿瘤作用 |
| Synergistic Antitumor Effect of Deguelin Combined with 5-Fluorouracil on Gastric Carcinoma Cell Line MGC-803 |
| 投稿时间：2013-01-16 |
| DOI：10.11735/j.issn.1671-170X.2013.07.B005 |
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| 中文关键词: 鱼藤素 氟尿嘧啶 胃肿瘤 协同作用 |
| 英文关键词:deguelin 5-Fluorouracil gastric neoplasms chemosensitization |
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| 中文摘要: |
| 摘 要：［目的］ 观察鱼藤素与氟尿嘧啶联用对胃癌细胞的作用。［方法］ 胃癌MGC-803细胞经鱼藤素、氟尿嘧啶单药及两药联合处理48h后，MTT法检测其对细胞生长增殖抑制水平，采用等效图解法分析其作用；流式细胞术检测经鱼藤素处理后MGC-803细胞周期的变化。［结果］ 鱼藤素可剂量依赖性抑制MGC-803细胞增殖，有G1期阻滞作用，与氟尿嘧啶联用可明显增强细胞杀伤作用。［结论］ 鱼藤素与氟尿嘧啶联用可产生明显协同作用，机制为两者作用于不同细胞周期，增强了细胞对氟尿嘧啶的敏感性。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the effects of deguelin combined with 5-Fluorouracil on gastric carcinoma cells. ［Methods］ Human gastric carcinoma cell line MGC-803 was used in present study. The inhibition effect on cell proliferation was determined by the MTT assay 48h after treated with deguelin，5-Fluorouracil and the combination. The results of MTT assays were analyzed by isobologram. Cell cycle distribution was determined by flow cytometry after treated with deguelin. ［Results］ Deguelin inhibited proliferation of MGC-803 cells in dose-dependent manner. After treated with deguelin，the cell cycle was arrested in G1 phase. The killing effect of 5-Fluorouracil to MGC-803 cells markedly increased after combination with deguelin. ［Conclusion］ Combination of deguelin and 5-Fluorouracil exhibits synergistic effects on MGC-803 cells because the two drugs act on different cell cycle phase. |
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