| 窦征岳,江其生.阿霉素诱导人肺癌A549细胞凋亡及其免疫原性研究[J].肿瘤学杂志,2013,19(6):423-426. |
| 阿霉素诱导人肺癌A549细胞凋亡及其免疫原性研究 |
| Adriamycin Induced Apoptosis in Human Lung Adenocarcinoma Cell Lines A549 and Immunogenicity of Apoptosis Cells |
| 投稿时间：2012-11-07 |
| DOI：10.11735/j.issn.1671-170X.2013.06.B003 |
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| 中文关键词: 肺肿瘤 细胞凋亡 阿霉素 免疫原性 免疫治疗 |
| 英文关键词:lung neoplasms cell apoptosis adriamycin immunogenicity immunotherapy |
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| 摘要点击次数: 1860 |
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| 中文摘要: |
| 摘 要：［目的］ 研究阿霉素诱导的人肺癌A549细胞凋亡及免疫原性。［方法］ 体外培养人肺癌A549细胞，将阿霉素加入A549细胞培养液中，使其终浓度分别为1mg/L、3mg/L、5mg/L，12h后以吖啶橙/溴化乙锭（AO/EB）双染色凋亡方法检测其诱导的细胞凋亡情况；以凋亡的A549细胞为抗原，体外致敏分离的正常人外周血单个核细胞，72h后以MTT法检测其对培养的A549细胞的杀伤效果。［结果］ 阿霉素按1mg/L、3mg/L、5mg/L终浓度作用A549细胞12h后，凋亡率分别为17.60%±1.77%，62.93%±1.70%，16.80%±0.65%。在外周血单个核细胞杀伤实验中，阿霉素诱导的A549凋亡细胞抗原组（A组）光密度（OD）值为0.66508±0.036859，正常细胞对照组（N组）OD值为0.94132±0.018455，凋亡抑制组（Z-A组）为0.71810±0.026841。A组与N组、Z-A组相比，OD值均显著降低（P<0.05）。［结论］ 阿霉素可诱导A549细胞凋亡，而由凋亡细胞致敏的体外单个核细胞可产生对A549细胞的杀伤效应。 |
| 英文摘要: |
| Abstract:［Purpose］ To study adriamycin(APM) induced apoptosis in lung adenocarcinoma A549 cell lines and the immunogenicity of apoptosis cells. ［Methods］ A549 cells were cultivated with ADM in vitro. After 12h cultivation，apoptosis ratio with ADM concentration of 1mg/L，3mg/L，5mg/L was determined by acridine orange/ethidium bromide(AO/EB) double dye measurement. With apoptosis A549 cells as antigen，peripheral blood mononuclear cell (PBMC) were sensitized，and after 72h，killing effect was detected by MTT.［Results］ After treated with ADM at concentration of 1mg/L，3mg/L，5mg/L，apoptosis rate of A549 was 17.6%±1.77%，62.93%±1.70%，16.8%±0.65% respectively. In peripheral blood mononuclear cell killing experiments，optical density (OD) in group of antigen maked by apoptotic A549 cells induced by adriamycin (group A) was 0.66508±0.036859，and that in normal cell group (group N) and in apoptosis inhibited group(group Z-A) was 0.94132±0.018455 and 0.71810±0.026841 respectively. Comparing group A with group N and with group Z-A，OD of group A was significantly lower than that of group N or group Z-A(P<0.05).［Conclusion］ ADM is capcable of inducing cell apoptosis in A549 cell line，and PBMC sensitized by apoptosis cells was provided with killing effect to A549 cells. |
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