| 饶远权,刘杏娥,王廷祥.人参皂苷Rg3抑制肺癌NCI-H1650细胞增殖作用研究[J].肿瘤学杂志,2013,19(6):413-416. |
| 人参皂苷Rg3抑制肺癌NCI-H1650细胞增殖作用研究 |
| Ginsenoside Rg3 Inhibiting Proliferation of Lung Cancer NCI-H1650 Cells |
| 投稿时间：2013-03-19 |
| DOI：10.11735/j.issn.1671-170X.2013.6.B001 |
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| 中文关键词: 人参皂苷 肺肿瘤 细胞凋亡 |
| 英文关键词:ginsenoside lung neoplasms cell apoptosis |
| 基金项目:浙江省中医药科技计划基金资助项目（2013ZA014） |
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| 中文摘要: |
| 摘 要:［目的］ 研究人参皂苷Rg3对肺腺癌NCI-H1650细胞增殖的抑制作用及对凋亡的影响。［方法］ MTT法检测人参皂苷Rg3对NCI-H1650细胞增殖的抑制作用，Annexin-V-FITC/PI法检测细胞凋亡，Western blot法检测caspase-3、Bcl-2、Bax、survivin蛋白表达的情况。［结果］ 不同浓度人参皂苷Rg3能有效抑制NCI-H1650细胞增殖，且呈时间剂量依赖性（P＜0.01）。20μmol/L人参皂苷作用NCI-H1650细胞24、48h，细胞凋亡率明显增加，与对照组相比，差异有统计学意义（P＜0.01）。20μmol/L人参皂苷作用NCI-H1650细胞48h，Bcl-2、survivin蛋白表达降低、caspase-3、Bax蛋白表达增加，与对照组相比，差异具有统计学意义（P＜0.01）。［结论］ 人参皂苷Rg3对肺腺癌NCI-H1650细胞增殖具有明显的抑制作用，呈时间剂量依赖关系，其机制可能与降低细胞Bcl-2、survivin蛋白表达，增加caspase-3、Bax蛋白表达，促进细胞凋亡有关。 |
| 英文摘要: |
| Abstract:［Purpose］To investigate ginsenoside Rg3 inhibiting proliferation of lung cancer NCI-H1650 cells and its influence on cell apoptosis.［Methods］ Inhibitive effect of ginsenoside Rg3 on proliferation of lung cancer NCI-H1650 cells was detected by MTT assay，and cell apoptosis was detected by Annexin-V-FITC/PI assay，expressions of caspase-3，Bcl-2，Bax，survivin proteins were detected by Western blot.［Results］ Ginsenoside Rg3 with various concentrations effectively inhibited the proliferation of NCI-H1650 cells with a time-dose dependent manner (P<0.01). Compared with the control group，ginsenoside Rg3 in a concentration of 20μmol/L significantly increased the apoptosis rate of NCI-H1650 cells in 24h and 48h (P<0.01). Compared with the control group，expressions of Bcl-2 and survivin decreased after NCI-H1650 cells treated with ginsenoside Rg3 in a concentration of 20μmol/L for 48h，with significant difference between the two groups (P<0.01).［Conclusion］ Ginsenoside Rg3 could significantly inhibite the proliferation of NCI-H1650 cells with time-dose dependent manner，and the mechanism may be related to reduce Bcl-2，survivin proteins’ expressions，increase caspase-3，Bax proteins’ expressions，promote cell apoptosis. |
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