李 波,凌志强,郑 吉.食管鳞癌患者外周血MMP-9 mRNA表达水平及临床意义研究[J].肿瘤学杂志,2013,19(5):352-356.
食管鳞癌患者外周血MMP-9 mRNA表达水平及临床意义研究
Expression of Matrix Metalloproteinase-9 (MMP-9) mRNA in the Peripheral Blood of Patients with Esophageal Squamous Cell Cancer and Its Clinical Significance
投稿时间:2013-03-27  
DOI:10.11735/j.issn.1671-170X.2013.05.B201303129
中文关键词:  食管肿瘤  鳞状细胞癌  基质金属蛋白酶-9(MMP-9)  实时定量PCR  外周血
英文关键词:esophageal neoplasms  squamous cell carcinoma  matrix metalloproteinase-9(MMP-9)  real-time qPCR  peripheral blood
基金项目:浙江省重大科技专项计划项目(2011C13039-1);卫生部科学研究基金——浙江省医药卫生重大科技计划项目(WKJ2011-2-015);浙江省医药卫生平台重点资助计划(2011ZDA004)
作者单位
李 波 浙江省中医药大学2010级硕士研究生 
凌志强 浙江省肿瘤医院浙江省肿瘤研究所 
郑 吉 浙江省中医药大学2010级硕士研究生 
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中文摘要:
      摘 要:[目的] 探讨食管鳞癌患者外周血中基质金属蛋白酶-9(MMP-9)基因的表达及其与临床病理资料、生存情况的关系。[方法] 应用实时定量PCR技术定量检测食管癌外周血标本中MMP-9 mRNA的表达,分析MMP-9基因表达情况与患者临床病理参数及预后之间的关系。[结果] 在169例食管鳞癌患者外周血标本中,有106例(62.7%)存在外周血MMP-9基因的阳性表达,与食管癌的淋巴结转移(P=0.039)、远处转移(P=0.019)和TNM分期(P=0.014)显著相关。单因素生存分析显示N分期(P=0.000)、M分期(P=0.002)、TNM分期(P=0.003)、MMP-9表达 (P=0.042)对患者生存的影响有统计学意义。MMP-9基因阳性表达的食管癌患者远期生存明显低于阴性表达者。多因素分析显示N分期是食管鳞癌患者预后的独立影响因子(RR=1.671,95%CI:1.212~2.306)。[结论] 食管鳞癌患者外周血中MMP-9基因的高表达与食管癌转移存在关联,并可提示食管癌患者的不良预后。
英文摘要:
      Abstract:[Purpose] To investigate the expression of matrix metalloproteinase-9(MMP-9) gene in the peripheral blood of patients with esophageal squamous cell cancer(ESCC) and its relationships with clinicopathologic characteristics and survival.[Methods] The expression of MMP-9 mRNA from peripheral blood samples of ESCC was detected by real-time qPCR,and the relationships of MMP-9 expression with clinicopathologic characteristics and prognosis were analyzed.[Results] The positive expression rate of MMP-9 in the peripheral blood of patients with ESCC was 62.7%(106/169),which positively correlated with the lymph node metastasis (P=0.039),distant metastasis (P=0.019) and TNM stage(P=0.014)。Univariate analysis showed that N stage (P=0.000),M stage(P=0.002),TNM stage(P=0.003) and MMP-9 expression (P=0.042) had significant impact on patients’ survival. The survival of ESCC patients with positive expression of MMP-9 was lower than that of patients with negative expression of MMP-9.Multivariate analysis showed that N stage was an independent impact factor for prognosis(RR=1.671,95%CI:1.212~2.306).[Conclusion] The high expression of MMP-9 correlates with the metastasis of esophageal cancer and might indicate poor prognosis.
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