| 李 波,凌志强,郑 吉.食管鳞癌患者外周血MMP-9 mRNA表达水平及临床意义研究[J].肿瘤学杂志,2013,19(5):352-356. |
| 食管鳞癌患者外周血MMP-9 mRNA表达水平及临床意义研究 |
| Expression of Matrix Metalloproteinase-9 (MMP-9) mRNA in the Peripheral Blood of Patients with Esophageal Squamous Cell Cancer and Its Clinical Significance |
| 投稿时间：2013-03-27 |
| DOI：10.11735/j.issn.1671-170X.2013.05.B201303129 |
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| 中文关键词: 食管肿瘤 鳞状细胞癌 基质金属蛋白酶-9（MMP-9） 实时定量PCR 外周血 |
| 英文关键词:esophageal neoplasms squamous cell carcinoma matrix metalloproteinase-9(MMP-9) real-time qPCR peripheral blood |
| 基金项目:浙江省重大科技专项计划项目（2011C13039-1）；卫生部科学研究基金——浙江省医药卫生重大科技计划项目（WKJ2011-2-015）；浙江省医药卫生平台重点资助计划（2011ZDA004） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨食管鳞癌患者外周血中基质金属蛋白酶-9（MMP-9）基因的表达及其与临床病理资料、生存情况的关系。［方法］ 应用实时定量PCR技术定量检测食管癌外周血标本中MMP-9 mRNA的表达，分析MMP-9基因表达情况与患者临床病理参数及预后之间的关系。［结果］ 在169例食管鳞癌患者外周血标本中，有106例（62.7%）存在外周血MMP-9基因的阳性表达，与食管癌的淋巴结转移（P=0.039）、远处转移(P=0.019)和TNM分期(P=0.014)显著相关。单因素生存分析显示N分期(P=0.000)、M分期（P=0.002）、TNM分期（P=0.003）、MMP-9表达 (P=0.042)对患者生存的影响有统计学意义。MMP-9基因阳性表达的食管癌患者远期生存明显低于阴性表达者。多因素分析显示N分期是食管鳞癌患者预后的独立影响因子（RR=1.671，95%CI:1.212～2.306）。［结论］ 食管鳞癌患者外周血中MMP-9基因的高表达与食管癌转移存在关联，并可提示食管癌患者的不良预后。 |
| 英文摘要: |
| Abstract:［Purpose］ To investigate the expression of matrix metalloproteinase-9(MMP-9) gene in the peripheral blood of patients with esophageal squamous cell cancer(ESCC) and its relationships with clinicopathologic characteristics and survival.［Methods］ The expression of MMP-9 mRNA from peripheral blood samples of ESCC was detected by real-time qPCR，and the relationships of MMP-9 expression with clinicopathologic characteristics and prognosis were analyzed.［Results］ The positive expression rate of MMP-9 in the peripheral blood of patients with ESCC was 62.7%(106/169)，which positively correlated with the lymph node metastasis (P=0.039)，distant metastasis (P=0.019) and TNM stage(P=0.014)。Univariate analysis showed that N stage (P=0.000)，M stage（P=0.002），TNM stage（P=0.003） and MMP-9 expression (P=0.042) had significant impact on patients’ survival. The survival of ESCC patients with positive expression of MMP-9 was lower than that of patients with negative expression of MMP-9.Multivariate analysis showed that N stage was an independent impact factor for prognosis（RR=1.671，95%CI:1.212～2.306）.［Conclusion］ The high expression of MMP-9 correlates with the metastasis of esophageal cancer and might indicate poor prognosis. |
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