| 吴敏云,胡 芝,张 晶.miR-143及其前体转录本在宫颈鳞癌组织中的表达[J].肿瘤学杂志,2013,19(5):373-377. |
| miR-143及其前体转录本在宫颈鳞癌组织中的表达 |
| Expression of miR-143 and Its Primary Transcript in Squamous Cell Carcinoma of Cervix |
| 投稿时间：2013-03-04 |
| DOI：10.11735/j.issn.1671-170X.2013.05.B201303089 |
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| 中文关键词: microRNA miR-143 宫颈肿瘤 鳞状细胞癌 |
| 英文关键词:microRNA miR-143 cervical neoplasms squamous cell carcinoma |
| 基金项目:温州市科技计划项目（Y20100255） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-143及其前体转录本在宫颈鳞癌中的表达及与临床病理特征的关系。［方法］ 采用Real-time PCR方法检测48例宫颈鳞癌及20例正常宫颈组织中miR-143的表达，分析miR-143与其前体转录本表达间的相关性，以及miR-143表达与宫颈鳞癌临床病理特征的关系。［结果］ 用Real-time PCR能有效检测miR-143及其前体转录本；miR-143及其前体转录本在宫颈鳞癌组织中的表达均显著低于正常宫颈组织（P＜0.05），Spearman相关分析显示，miR-143前体转录本表达和miR-143表达呈明显正相关（r=0.723，P=0.000）。不同组织分化程度的宫颈鳞癌miR-143表达存在显著差异（P=0.005），小细胞型宫颈癌的miR-143表达明显低于其他组织分化类型的标本。miR-143的表达在不同年龄、肿块大小、大体类型及FIGO分期间未见显著差异（P>0.05）。［结论］ 前体转录本减少是miR-143表达下调的重要原因。miR-143在宫颈鳞癌组织中的异常表达，可能在宫颈鳞癌的发生和发展过程中发挥重要作用，有望成为宫颈鳞癌新的预后指标及治疗靶点。 |
| 英文摘要: |
| Abstract:［Purpose］ To investigate the expressions of miR-143 and its primary transcript (pri-miR-143) in squamous cell carcinoma of cervix and the relationship of miR-143 with clinicopathologic features of squamous cell carcinoma of cervix.［Methods］ The expressions of miR-143 in 48 cases with squamous cell carcinoma of cervix and 20 normal cervical tissue specimens were examined by real-time PCR. The correlation between the expressions of miR-143 and pri-miR-143 was analyzed. The relationship of the expression of miR-143 and clinicopathologic features of squamous cell carcinoma were explored. ［Results］ The real-time PCR could sensitively and specifically detect the expressions of miR-143 and pri-miR-143. Expressions of miR-143 and pri-miR-143 in squamous cell carcinoma of cervix were obviously lower than those in normal cervical tissue（P＜0.05). Spearman correlation analysis showed that the expression of miR-143 was positively associated with the expression of pri-miR-143（r=0.723，P=0.000）. The level of miR-143 was significantly different in squamous cell carcinoma of cervix with different histological grades(P=0.005）. The expressions of miR-143 in small cell type of squamous cell carcinoma of cervix was significantly lower than that in the other type one. No significant difference was found in miR-143 expression in squamous cell carcinoma of cervix with ages，tumor sizes，general types，and FIGO stages（P>0.05）. ［Conclusion］ Primary transcription reduction is an important reason for down-regulation of miR-143 expression. miR-143 might play an important role in the carcinogensis and progress of squamous cell carcinoma of cervix and may be a novel potential therapeutic target for squamous cell carcinoma of cervix. |
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