| 奚海林,陶凯雄,牛建华.慢病毒介导RNAi抑制胃癌细胞CDH17基因的表达对化疗药物敏感性的影响[J].肿瘤学杂志,2013,19(5):321-326. |
| 慢病毒介导RNAi抑制胃癌细胞CDH17基因的表达对化疗药物敏感性的影响 |
| Lentivirus-mediated RNA Interference Suppresses CDH17 Expression and Enhances Chemosensitivity in Gastric Cancer Cells |
| 投稿时间：2013-01-04 |
| DOI：10.11735/j.issn.1671-170X.2013.05.B201301002 |
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| 中文关键词: 胃肿瘤 CDH17 细胞增殖 RNA干扰 氟尿嘧啶 |
| 英文关键词:Stomach neoplasms CDH17 gene cell proliferation RNA interference 5-FU |
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| 中文摘要: |
| 摘 要：［目的］ 探讨慢病毒介导的RNA干扰（RNAi) 沉默肝肠—钙黏连蛋白(CDH17)基因的胃癌细胞SGC-7901对化疗药物敏感性的影响。［方法］ 将制备好的siRNA-CDH17慢病毒表达载体稳定转染SGC-7901细胞72h后，采用实时定量PCR法检测转染后SGC-7901细胞中CDH17基因的表达水平；Western blot检测CDH17蛋白的表达水平；利用流式细胞仪检测细胞周期，MTT法和平板克隆形成实验检测CDH17基因沉默后胃癌细胞SGC-7901对氟尿嘧啶(5-FU)的敏感性。［结果］ Real-time PCR及Western blot检测结果表明CDH17 mRNA及蛋白在SGC-7901细胞中表达下调；实验组SGC-7901细胞周期明显阻滞在G0/G1期，S期相对减少；5-FU 处理SGC-7901细胞48h后，MTT法和平板克隆形成实验显示实验组增殖抑制显著高于另两组，差异有统计学意义(P<0.001)。CDH17基因沉默的SGC-7901细胞对5-FU的敏感性显著增强。［结论］ 以CDH17基因为靶标的RNA干扰能下调SGC-7901细胞中CDH17的表达，从而增强其对5-FU的化疗敏感性。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the effects of lentivirus-mediated RNA interference (RNAi) targeting CDH17 (liver-intestine cadherin) on the chemosensitivity to 5-FU of gastric cancer cell line SGC-7901. ［Methods］ SGC-7901 cells were steady transfected with lentiviruses delivering small interfering RNA (siRNA) against CDH17 gene. The expression levels of CDH17 mRNA and protein were detected by real-time PCR and Western blot，respectively. Flow cytometry was used to detect the cell cycle. Sensitivity to 5-FU after transfection were examined by MTT and colony formation assay. ［Results］ In SGC-7901 cells，the expression levels of CDH17 mRNA and protein decreased significantly after transfection. The cell number in G0/G1 phase increased while that in S phase decreased in experimental group cells. Forty-eight hours after treatment with 5-FU，the growth inhibition rate in experimental group was significantly higher than that in the other two groups (P<0.001). The sensitivity of SGC-7901 cells to 5-FU increased significantly after transfection. ［Conclusion］ Lentivirus-mediated RNAi targeting CDH17 can effectively suppress the expression of CDH17 in SGC-7901 cells and enhance the chemosensitivity of the cells to 5-FU. |
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