| 李学成,卢 斌,王宗华.胃癌组织内miR-29a与VEGF-A表达的相关性研究[J].肿瘤学杂志,2013,19(1):38-41. |
| 胃癌组织内miR-29a与VEGF-A表达的相关性研究 |
| Expression of miR-29 and Its Relationship with Vascular Endothelial Growth Factor-A(VEGF-A) in Gastric Cancer |
| 投稿时间：2012-10-19 |
| DOI：10.11735/j.issn.1671-170X.2013.01.B2012530 |
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| 中文关键词: 胃肿瘤 miR-29a 血管内皮生长因子-A |
| 英文关键词:gastric neoplasms miR-29a vascular endothelial growth factor-A(VEGF-A) |
| 基金项目:重庆市自然科学基金（CSTC2010BB5158；CSTC2011AC5024； CSTC2011jjA0878；CSTC2011jjA0882；CSTC2012jjA1736） |
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| 中文摘要: |
| 摘 要：［目的］ 观察miR-29a和血管内皮生长因子-A（VEGF-A）在胃癌病人血清及组织中的表达，分析两者表达相关性。［方法］ 采用荧光实时定量PCR技术检测50例胃癌患者血清中miR-29a，采用ELISA技术检测血清VEGF-A的表达，利用免疫组化法检测113例胃癌组织（57例miR-29a高表达组及56例miR-29a低表达组）中VEGF-A的表达，并分析两者表达相关性；利用生物信息学预测miR-29a与VEGF-A 3′UTR结合位点。［结果］ 胃癌患者血清中miR-29a与VEGF-A的表达呈现明显负相关（R2=0.56，P<0.0001）；胃癌组织中miR-29a低表达组VEGF-A表达阳性率明显高于miR-29a高表达组（74.32%±7.98% vs 34.37%±6.59%，P<0.001）；VEGF-A 3′UTR序列可能有miR-29a稳定的结合位点。［结论］ miR-29a是一个潜在的抑癌miRNA，可能是通过抑制VEGF-A的表达而发挥抑癌作用。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the expression of miR-29a and its relationship with vascular endothelial growth factor-A(VEGF-A) in gastric cancer tissues and serum. ［Methods］ The expression of serous miR-29a and VEGF-A in 50 cases with gastric cancer were detected by real-time PCR and ELISA，respectively. The expression of VEGF-A in 113 gastric cancer tissues (57 cases with miR-29a high expression，56 cases with miR-29a low expression) were detected immunohistochemically，and the expressions between serous and tissues miR-29a or VEGF-A in gastric cancer were analyzed. The miR-29a binding site on VEGF-A 3’UTR sequence was analyzed by bioinformatic predictions program. ［Results］ The expression of miR-29a and VEGF-A in the serum of patients with gastric cancer was significantly negative correlation(R2=0.56，P<0.0001). The expression of VEGF-A in low miR-29a expression group of gastric cancer tissues was significantly higher than that in high miR-29a expression group (74.32%±7.98% vs 34.37%±6.59%，P<0.001). VEGF-A 3’UTR sequence might have stable binding site with miR-29a.［Conclusion］ By suppressing VEGF-A expression，miR-29a is probably a potential tumor suppressing miRNA. |
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