余南荣,李昌宾,纪术峰.小白菊内酯及顺铂对人结肠癌细胞SW620凋亡和增殖的作用[J].肿瘤学杂志,2013,19(4):277-280. |
小白菊内酯及顺铂对人结肠癌细胞SW620凋亡和增殖的作用 |
The Effect of Parthenolide and Cisplatin on Proliferation and Apoptosis in Human Colon Cancer Cell SW620 |
投稿时间:2012-10-15 |
DOI:10.11735/j.issn.1671-170X.2013.04.B2012517 |
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中文关键词: 小白菊内酯 顺铂 SW620细胞 细胞增殖 细胞凋亡 |
英文关键词:parthenolide cisplatin SW620 cell proliferation apoptosis |
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中文摘要: |
摘 要:[目的] 探讨小白菊内酯及顺铂对人结肠癌细胞SW620增殖及凋亡的影响。[方法] 不同浓度的小白菊内酯和顺铂分别处理SW620细胞,MTT法检测细胞增殖的变化情况,流式细胞仪检测细胞凋亡的情况。[结果] 小白菊内酯和顺铂对SW620细胞增殖均具有抑制作用;分别作用48h后,小白菊内酯和顺铂对SW620细胞增殖的抑制作用呈浓度依赖性;5μmol/L顺铂联合10μmol/L小白菊内酯作用SW620细胞时,对细胞增殖具有协同抑制作用。小白菊内酯和顺铂可以诱导SW620细胞凋亡。[结论] 小白菊内酯和顺铂联用具有协同作用,能抑制SW620细胞增殖并诱导细胞凋亡。 |
英文摘要: |
Abstract:[Purpose] To investigate the effect of parthenolide and(or) cisplatin on proliferation and apoptosis in human colon cancer cell SW620. [Methods] After 48h treated by parthenolide and(or) cisplatin with different concentrations,the proliferation of SW620 cells was determined by MTT assay,and the cell apoptosis was examined by flow cytometry. [Results] Parthenolide and cisplatin were capable of inhibiting SW620 cells proliferation,and the inhibition was dose-dependent.Parthenolide 5μmol/L and cisplatin 10μmol/L synergistically inhibited the proliferation of SW620 cells. The cell apoptosis also could be induced by parthenolide and(or) cisplatin. [Conclusion] Parthenolide and cisplatin have synergistical effects on inhibiting cell proliferation and inducing cell apoptosis of SW620 cells. |
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