| 李 青,汪 艳,朱慧芬.内皮素-1促进CD133+卵巢肿瘤干细胞血管新生的机制研究[J].肿瘤学杂志,2013,19(2):85-89. |
| 内皮素-1促进CD133+卵巢肿瘤干细胞血管新生的机制研究 |
| Study on Endothelin-1(ET-1) Contributing to Angiogenesis of CD133+ Ovarian Cancer Stem Cells |
| 投稿时间：2012-12-16 |
| DOI：10.11735/j.issn.1671-170X.2013.02.B2012504 |
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| 中文关键词: CD133+ 肿瘤干细胞 血管生成 缺氧 内皮素1（ET-1） 缺氧诱导因子1α（HIF-1α） 血管内皮生长因子（VEGF） |
| 英文关键词:CD133+ cancer stem cell angiogenesis hypoxia endothelin-1(ET-1) hypoxia-inducible factor 1α(HIF-1α) vascular endothelial growth factor(VEGF) |
| 基金项目:安徽省2010年度第四批科技计划项目（10020503083） |
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| 中文摘要: |
| 摘 要：［目的］ 研究内皮素-1(ET-1)促进CD133+肿瘤干细胞血管新生的机制。［方法］ ET-1作用CD133+肿瘤干细胞48h后，通过ELISA法检测细胞内血管内皮生长因子(VEGF)表达量；Northern blot 检测 VEGF mRNA 表达量；Western blot 检测缺氧诱导因子-1α(HIF-1α)含量。［结果］ 在正常条件和缺氧条件下，经ET-1处理的细胞VEGF表达明显高于对照组（P<0.05）；BQ123+ET-1组细胞内VEGF mRNA量明显低于ET-1组（P<0.05）；经ET-1处理后，细胞从缺氧转到正常条件下，细胞内HIF-1α稳定性增强。［结论］ ET-1通过其受体A(ETAR)介导CD133+细胞的VEGF表达上调；降低HIF-1α被蛋白酶降解速度，稳定HIF-1α的表达，从而促进CD133+肿瘤干细胞血管新生。 |
| 英文摘要: |
| Abstract:［Purpose］ To investigate the mechanism of angiogenesis of CD133+ cancer stem cells promoted by endothelin-1(ET-1).［Methods］ After CD133+ cancer stem cells was treated by ET-1 for 48h，VEGF and HIF-1α were detected by ELISA and Western blot respectively. Northern blot was explored to evaluate the expression of VEGF mRNA. ［Results］ The expression of VEGF in cells treated with ET-1 was obviously higher than that of the control group under normal and hypoxia condition(P<0.05). But the expression of VEGF mRNA of cells treated with BQ123 and ET-1 was reduced compared to cells treated with ET-1(P<0.05). After treatment with ET-1，HIF-1α was stabilized. ［Conclusion］ ET-1 contributes to the expression of VEGF in CD 133+ cancer stem cells via ETAR receptor. It also slows down degradation velocity of HIF-1α by protease，stabilizes the expression of HIF-1α，which could stimulate angiogenesis of CD133+ cancer stem cells. |
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