| 赵 群,吴玉梅,王建东.实时荧光定量 PCR检测早期宫颈癌及前哨淋巴结VEGF-C及VEGF-D mRNA表达[J].肿瘤学杂志,2013,19(1):14-18. |
| 实时荧光定量 PCR检测早期宫颈癌及前哨淋巴结VEGF-C及VEGF-D mRNA表达 |
| Detection of VEGF-C and VEGF-D mRNA in Cervical Cancer Early Stage and Sentinel Lymphatic Node by Real-Time PCR |
| 投稿时间：2012-07-06 |
| DOI：10.11735/j.issn.1671-170X.2013.01.B2012324 |
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| 中文关键词: 宫颈肿瘤 前哨淋巴结 血管内皮生长因子（VEGF） 实时荧光定量PCR |
| 英文关键词:cervical neoplasms sentinel lymph nodes vascular endothelial growth factor(VEGF) real?鄄time PCR |
| 基金项目:北京市卫生局科学研究基金 |
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| 中文摘要: |
| 摘 要：［目的］ 研究血管内皮生长因子（VEGF）-C及VEGF-D mRNA在早期宫颈癌和前哨淋巴结中的表达水平。［方法］ 收集51例早期宫颈癌患者宫颈癌组织、癌周组织、前哨转移淋巴结、非前哨无转移淋巴结、正常区域组织。透射电镜观察淋巴转移情况，实时荧光定量PCR检测VEGF-C及VEGF-D mRNA的表达量，并研究其与宫颈癌临床病理各因素之间的关系。［结果］ 透射电镜显示宫颈癌周区可见到淋巴管增生。VEGF-C及VEGF-D mRNA在宫颈癌内、癌周、前哨转移淋巴结中的表达均显著高于正常宫颈组织和非前哨无转移淋巴结（P<0.05）。VEGF-C及VEGF-D mRNA在宫颈癌组织中的表达在不同淋巴结转移、淋巴管侵犯、肿瘤浸润深度下差异有统计学意义（P均<0.05），而不同年龄、临床分期、病灶大小、组织分化、病理类型之间的差异无统计学意义（P均>0.05）。［结论］ VEGF-C及VEGF-D在早期宫颈癌的淋巴结转移中起重要作用，可能成为预测宫颈癌淋巴结转移的关键指标，有希望成为抑制宫颈癌淋巴结转移的治疗靶点。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate VEGF-C and VEGF-D mRNA levels in early stage of cervical cancer and sentinel lymphatic nodes(SLN). ［Methods］ The cancer tissues，pericancerous tissues，positive SLN，negative non-SLN and normal tissues in 51 cases with cervical cancer early stage were collected. Lymphatic metastasis was observed using transmission electronic microscope，VEGF-C and VEGF-D mRNA were detected by real-time PCR，and the relationships of VEGF-C and VEGF-D mRNA with clinicopathologic factors were studied. ［Results］ With transmission electronic microscopy，there were hyperplastic lymphatic vessels surrounding the cervical cancer tissues. Expressions of VEGF-C and VEGF-D mRNA in cancer tissues，pericancerous tissues，or positive SLN tissues were significantly higher than those in negative non-SLN or normal tissues（P<0.05）. The expressions of VEGF-C and VEGF-D mRNA in cervical cancer were significantly different between those with and without lymph node metastasis，those with and without lymph vessel invasion，and depthes of tumor invasion（P<0.05），but there was no significant difference between ages，stages，lesion sizes，grades，or pathologic types（P>0.05）. ［Conclusion］ VEGF-C and VEGF-D play an important role in lymph node metastasis of cervical cancer early stage. VEGF-C and VEGF-D may be crucial indexes for predicting lymph nodes metastasis，and may become treatment target for inhibition of lymph node metastasis of cervical cancer. |
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