| 徐国森,林海峰,佟丹丹.荧光原位杂交与免疫组织化学方法对乳腺癌Her-2检测的比较[J].肿瘤学杂志,2012,18(12):939-943. |
| 荧光原位杂交与免疫组织化学方法对乳腺癌Her-2检测的比较 |
| Comparison of Her-2 Detections by Fluorescence in situ Hybridization(FISH) and by Immunohistochemistry (IHC) in Breast Cancer |
| 投稿时间：2012-10-13 |
| DOI： |
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| 中文关键词: 乳腺肿瘤 荧光原位杂交 人类表皮生长因子受体 免疫组织化学 多倍体 |
| 英文关键词:breast neoplasms fluorescent in situ hybridization human epithelium growth factor receptor immunohistochemistry polyploidy |
| 基金项目:黑龙江省自然基金(D200921)；哈尔滨医科大学附属第三医院院内基金。 |
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| 中文摘要: |
| 摘 要: ［目的］ 探讨荧光原位杂交(FISH)与免疫组织化学方法(IHC)检测乳腺癌组织中人类表皮生长因子受体2(Her-2)表达结果的差异。［方法］ 采用FISH和IHC法分别检测508例乳腺癌标本中Her-2基因扩增和Her-2蛋白表达状况。［结果］ 在IHC法检测Her-2蛋白表达0/+的104例(20.47%)标本中，FISH检出13例(2.56%)Her-2基因扩增，89例(17.52%)为非扩增，2例（0.39%）为可疑；Her-2蛋白表达++的245例（48.23%）标本中，FISH检出121例（23.82%）Her-2基因扩增，120例（23.62%）为非扩增，4例（0.79%）为可疑；IHC在Her-2蛋白表达+++的159例（31.30%）标本中，FISH检出137例（26.97%）Her-2基因扩增，22例（4.33%）为非扩增。［结论］对于IHC法初筛Her-2蛋白为++~+++的患者必须进行FISH检测，以明确Her-2基因的状态，从而指导临床医师诊断治疗。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the difference of Her-2 expression detected by fluorescence in situ hybridization(FISH) and by immunohistochemistry (IHC) in breast cancer. ［Methods］ Both IHC and FISH techniques were performed to detect Her-2 gene amplification and Her-2 protein expression respectively in 508 samples with breast cancer. ［Results］ Among 104 cases(20.47%) with 0/+ Her-2 protein expression detected by IHC，13 cases(2.56%) were Her-2 gene amplification detected by FISH;89 cases(17.52%)，non-amplification; 2 cases(0.39%)，suspicious. Among 245 cases(48.23%) with ++ Her-2 protein expression，121 cases(23.82%) were Her-2 gene amplification;120 cases(23.62%) ，non-amplification; 4 cases(0.79%)，suspicious. Among 159 cases(31.30%) with +++ Her-2 protein expression，137 cases(2.97%) were Her-2 gene amplification;22 cases(4.33%) ，non-amplification. ［Conclusion］ Detecting the amplification of Her-2 gene with FISH may be helpful to know Her-2 gene status for treatment decision in breast cancer patients with positive Her-2 expression ++~+++ by IHC. |
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