| 吴圣豪,郑翠苹,刘 珍.硼替佐米对K562细胞株DNMT1表达的影响[J].肿瘤学杂志,2012,18(12):920-922. |
| 硼替佐米对K562细胞株DNMT1表达的影响 |
| Effect of Bortezomib on the Expression of DNA Methyltransferase in K562 Cell Line |
| 投稿时间：2012-04-17 |
| DOI： |
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| 中文关键词: 白血病 硼替佐米 DNA甲基转移酶 |
| 英文关键词:leukemia Bortezomib DNA methyltransferase |
| 基金项目:温州市科技划项目（Y20100070） |
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| 摘要点击次数: 1351 |
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| 中文摘要: |
| 摘 要：［目的］探讨蛋白酶体抑制剂硼替佐米(bortezomib)对K562细胞株DNA甲基转移酶（DNMTs）表达、细胞凋亡的影响。［方法］ 以不同浓度的硼替佐米（0、6、20、60nmol/L）处理白血病K562细胞株，Western blot法检测细胞内DNMT1的表达，流式细胞术检测细胞凋亡。［结果］ 与对照组相比，硼替佐米可显著抑制DNMT1表达；硼替佐米作用于细胞12、24、36h后细胞凋亡率逐渐增加，60nmol/L硼替佐米作用36h后细胞凋亡率最高，为61.68%±3.20%。［结论］ 硼替佐米抑制K562细胞株DNMT1表达，并诱导细胞凋亡，呈浓度依赖性。 |
| 英文摘要: |
| Abstract:［Purpose］ To investigate the effects of bortezomib on the expression of DNA methyltransferase（DNMTs） and on cell apoptosis in K562 cell line．［Methods］ K562 cells were treated by bortezomib with the concentrations of 0，6，20 and 60nmol/L respectively. Western blot was used to analyze the intracellular expression of DNMT1，and flow cytometry was used to detect cell apoptosis. ［Results］ Compared with control group，the expression of DNMT1 decreased when treated with Bortezomib. Along with Bortezomib for 12，24，36h，the apoptosis increased gradually，and reached the highest after treating with 60nmol/L Bortezomib for 36h. ［Conclusion］ Bortezomib can suppress the expression of DNMT1 and induce apoptosis of K562 cells with concentration-dependence. |
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