| 陈佳萍,秦真真,曹松玉.miR-34b/c启动子区单核苷酸多态性对其转录调控的影响[J].肿瘤学杂志,2012,18(11):835-839. |
| miR-34b/c启动子区单核苷酸多态性对其转录调控的影响 |
| Ｉｍｐａｃｔ ｏｆ Single Nucleotide Polymorphism in the Promoter Region of miR-34b/c on Regulation of miR-34b/c Transcription |
| 投稿时间：2012-07-11 |
| DOI： |
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| 中文关键词: miR-34b/c 启动子区 单核苷酸多态性 |
| 英文关键词:miR-34b/c promoter region single nucleotide polymorphism |
| 基金项目:国家自然科学基金重点项目(30730080)；国家自然科学青年基金项目（81102179）。 |
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| 中文摘要: |
| 摘 要：［目的］ 研究miR-34b/c启动子区单核苷酸多态rs4938723 (T/C)对其转录调控的影响。［方法］ 构建含有不同基因型miR-34b/c启动子区的报告基因表达载体pGL3-basic-miR34b/c-T和pGL3-basic-miR34b/c-C，分别与内参质粒SV40共转染于Hela、A549和CHO三种细胞后进行荧光素酶活性分析。［结果］ pGL3-basic-miR34b/c-C等位基因组荧光素酶相对活性与pGL3-promoter-miR30C-T组等位基因相比，均有显著升高（P<0.01）。［结论］ 荧光素酶活性强弱间接反映了miRNA启动子区遗传变异对转录活性的影响，当miR-34b/c启动子区rs4938723位点为C时，可以更为有效地增加miR-34b/c的转录产物，从而在转录水平上调控miRNA的表达。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the ｉｍｐａｃｔ of a common polymorphism rs4938723 (T/C) in promoter region of miR-34b/c on the regulation of miR-34b/c transcription. ［Methods］ The pGL3-basic-miR34b/c-T and pGL3-basic-miR34b/c-C ｌuciferase plasmids were created containing T or C allele of miR-34b/c promoter region. Then the two plasmids contained T or C allele were transfected respectively into Hela，A549 and CHO cells to detect the transcription activity of miR-34b/c. ［Results］ pGL3-basic-miR34b/c-C construct group showed significantly higher levels of luciferase expression compared with pGL3-basic-miR34b/c-T construct group (P<0.01). ［Conclusion］ The miR34b/c transcript product could be effectively increased while the miR-34b/c carrying the rs4938723 C allele. |
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