| 杨雪飞,毛晓韵,范垂锋.香烟提取物对人非小细胞肺癌细胞系侵袭力及MTA1表达的影响[J].肿瘤学杂志,2012,18(11):801-805. |
| 香烟提取物对人非小细胞肺癌细胞系侵袭力及MTA1表达的影响 |
| The Effect of Cigarette Smoke Extract on Cell Invasion and MTA1 Expression in Non-small Lung Cancer Cell Lines |
| 投稿时间：2012-07-16 |
| DOI：10.11735/j.issn.1671-170X.2012.11.B001 |
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| 中文关键词: 癌，非小细胞肺 肿瘤转移相关基因1 香烟提取物 |
| 英文关键词:carcinoma, non-small cell lung metastasis tumor antigen 1 cigarette smoke extract |
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| 摘要点击次数: 1894 |
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| 中文摘要: |
| 摘 要:［目的］ 研究香烟提取物（CSE）对人非小细胞肺癌（NSCLC）细胞生物学行为的影响及可能机制。［方法］ CSE处理NSCLC细胞系A549、LH7与BE1，对照组正常培养。Transwell实验检测两组细胞的侵袭能力，RT-PCR法和Western blot法检测两组细胞MTA1 mRNA及蛋白表达情况。［结果］ CSE处理各CSE组细胞侵袭能力增加，穿过膜细胞数明显高于对照组；且各CSE组中MTA1 mRNA和蛋白表达水平也明显高于对照组（P<0.05）。［结论］ 香烟提取物导致NSCLC细胞系侵袭能力增加与其促进NSCLC细胞系中MTA1 mRNA及蛋白表达有关。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the effect of cigarette smoke extract (CSE) on the biological behaviour of non-small cell lung cancer (NSCLC) cell lines and the possible mechanism. ［Methods］ NSCLC cell lines A549，LH7 and BE1 were treated with CSE，and the control group was not treated. Cell invasive ability was examined by Transwell method. The MTA1 mRNA and protein expression were detected by RT-PCR and Western blot assay. ［Results］ CSE up-regulated the cell invasive ability，the number of migration cells of hypoxia in CSE group was higher than that in control group（P＜0.05）. The MTA1 mRNA and protein expression was up-regulated in the NSCLC cell lines with the treatment of CSE.The level was higher than that in control group（P＜0.05）. ［Conclusion］ The CSE can up-regulate the invasive ability of NSCLC cell lines，which correlates to MTA1 mRNA and its protein expression. |
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