| 英信江,董 频,徐宏鸣.HPV16E7-HSP70 融合蛋白在大肠杆菌中表达及纯化[J].肿瘤学杂志,2012,18(9):660-664. |
| HPV16E7-HSP70 融合蛋白在大肠杆菌中表达及纯化 |
| Expression and Purification of the HPV16E7-HSP70 Fusion Protein in Escherichia coli |
| 投稿时间：2012-07-11 |
| DOI： |
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| 中文关键词: 人乳头状瘤病毒 热休克蛋白 融合蛋白 包涵体 纯化 复性 大肠杆菌 |
| 英文关键词:human papillomavirus heat shock protein fusion protein inclusion body purification renaturation Escherichia coli |
| 基金项目:国家自然科学基金资助项目（3701610） |
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| 中文摘要: |
| 摘 要：［目的］ 在大肠杆菌中表达HPV16E7-HSP70 融合蛋白并进行纯化和复性，为进一步研究HPV16E7-HSP70融合蛋白抗喉癌免疫活性奠定基础。［方法］ 用构建的原核表达质粒pET28a HPV16E7-HSP70转化大肠杆菌BL21，异丙基-β-D-硫代半乳糖苷（IPTG）诱导蛋白表达；通过超声波裂解细菌，高浓度尿素裂解包涵体和Ni2+离子金属螯合亲和层析纯化目的蛋白；并对融合蛋白进行复性；用SDS-PAGE及Western Blot进行分析鉴定。［结果］ SDS-PAGE分析显示有分子量约为90kD的融合蛋白表达，表达产物以包涵体形式存在，表达量可达30%；纯化后目的蛋白的纯度达到95%；经Western Blot鉴定复性后的融合蛋白能与抗HPV16E7抗体、抗HSP70抗体特异性结合。［结论］ HPV16E7-HSP70 融合蛋白能够在体外大肠杆菌中表达获得。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the expression，purify and renature of HPV16E7-HSP70 fusion protein in Escherichia coli (E. coli) and to provide basis for further research. ［Methods］ The recombinant prokaryotic expression plasmid pET28a HPV16E7-HSP70 was transformed in E. coli BL21 and induced by isopropyl-β-D-thiogalactopyranoside(IPTG). After the bacterial pellet was lysed by sonication，solubilizied in ultrapure urea，the fusion protein expressed in E. coli was purified by Ni metal chelating chromatography following with renaturation，which was identified by SDS-PAGE and Western Blot analysis. ［Results］ SDS-PAGE analysis showed that the fusion protein with molecular weight of approximately 90 kD was expressed，which formed inclusion body in the cytoplasm of bacteria，with the ratio of target protein to total protein of host 30%. The purity up to 95% could be achieved after purification of target protein. Western Blot showed that the protein renatured could bind to HPV16E7 Ag and HSP70 Ag specifically.［Conclusion］ The HPV16E7-HSP70 fusion protein could be obtained in E. coli in vitro. |
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