Abstract:[Purpose] To investigate the value of radiocolloid combined with methylene blue dye in sentinel lymph node biopsy (SLNB) for patients with early breast cancer. [Methods] SLNB followed by axillary lymph node dissection (ALND) were carried out in 65 breast cancer patients,and SLN was detected by 99mTc-sulfur colliod and methylene blue. [Results] The success rate,accuracy rate and false negative rate of SLNB were 100.00%,98.46% and 6.25%,respectively. [Conclusions] Combination of radiocolliod and methylene blue tracer are practical in detecting sentinel lymph node,and it can effectively predict the metastases of axillary lymph node.