| 郑 彬,孙 峰.KDR基因沉默对胃癌MGC-803细胞增殖和凋亡的影响[J].肿瘤学杂志,2012,18(8):588-592. |
| KDR基因沉默对胃癌MGC-803细胞增殖和凋亡的影响 |
| Effect of KDR Gene Silence on Proliferation and Apoptosis of Gastric Cancer Cell Line MGC-803 |
| 投稿时间：2012-04-09 |
| DOI： |
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| 中文关键词: 胃肿瘤 KDR siRNA；细胞增殖；细胞凋亡 |
| 英文关键词:gastric neoplasmas KDR siRNA cell proliferation cell apoptosis |
| 基金项目: |
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| 摘要点击次数: 1836 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨KDR基因对胃癌MGC-803细胞增殖和凋亡的影响。［方法］ 设计及合成KDR的siRNA序列，LipofectamineTM 2000转染MGC-803细胞。通过RT-PCR、Western Blot检测KDR在干扰后的mRNA和蛋白的表达情况，利用流式细胞仪检测细胞周期，WST-1法检测细胞增殖活性，TUNEL法检测细胞凋亡情况。［结果］ KDR mRNA和蛋白表达，观察组较对照组和空白组显著降低，差异有统计学意义(P<0.05)。观察组细胞的生长速度明显减慢，细胞周期被阻滞在G0/G1期，S期细胞数减少，差异有统计学意义(P<0.05)。转染KDR siRNA 的MGC-803细胞的增殖能力明显受到抑制(P<0.05)，且明显促进了细胞的凋亡，差异有统计学意义(P<0.05)。［结论］ 特异性干扰KDR基因表达可抑制胃癌MGC-803细胞的增殖，并促进肿瘤细胞凋亡。KDR的siRNA序列可能成为治疗胃癌的有效靶点。 |
| 英文摘要: |
| Abstract: ［Purpose］ To explore the effect of KDR gene in the proliferation and apoptosis of gastric cancer cell MGC-803. ［Methods］ The siRNA of KDR was constructed and transfected into MGC-803 cells with LipofectamineTM 2000 . The expression of KDR mRNA and protein were detected by RT-PCR and Western Blot method. Flow cytometry was used to detect the cell cycle，the cell proliferation was assessed by WST-1 assay，and cell apoptosis was detected by TUNEL.［Results］ Compared with the control group and blank group，the mRNA and protein level was significantly decreased in the observation group when transfected with KDR siRNA. The growth slowed down and the cell cycle was arrested at G０/G1 phase and cell number in S phase was decreased in MGC-803 cell line (P>0.05).After KDR siRNA transfection，the proliferation of MGC-803 cells was markedly inhibited and the apoptosis of MGC-803 cells was increased (P<0.05).［Conclusion］ Interference of KDR gene may suppress cell growth and promote cell apoptosis in MGC-803 cell line.Thus，KDR siRNA might be an effective target spot in the treatment for gastric cancer. |
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