| 卢振铎,刘真真,王修身.丹酚酸乙对乳腺癌MCF-7细胞的生长抑制及诱导凋亡研究[J].肿瘤学杂志,2012,18(7):508-511. |
| 丹酚酸乙对乳腺癌MCF-7细胞的生长抑制及诱导凋亡研究 |
| The Effect of Salvianolic Acid B on Growth Inhibition and Apoptosis Induction in Breast Cancer MCF-7 Cells |
| 投稿时间：2012-06-04 |
| DOI： |
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| 中文关键词: 丹酚酸乙 MCF-7细胞 凋亡 caspase-3 乳腺肿瘤 |
| 英文关键词:salvianolic acid B MCF-7 cell apoptosis caspase-3 breast neoplasms |
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| 中文摘要: |
| 摘 要：［目的］ 观察丹酚酸乙对乳腺癌MCF-7细胞的生长抑制及诱导凋亡作用。［方法］ MTT法观察丹酚酸乙对体外培养MCF-7细胞的增殖抑制作用；Hoechst33258荧光染色法观察细胞凋亡的形态学变化；流式细胞仪检测丹酚酸乙作用于MCF-7细胞48h后细胞凋亡率和细胞周期的变化；Western Blot检测caspase-3蛋白表达的变化。［结果］ 丹酚酸乙能明显抑制MCF-7细胞的生长，呈剂量和时间依赖性；荧光染色结果显示，丹酚酸乙与MCF-7细胞共培养24、48和72h后，细胞呈现明显的核固缩、碎裂以及凋亡小体形成等细胞凋亡现象；流式细胞仪检测结果显示，不同浓度的丹酚酸乙处理MCF-7细胞48h后，细胞凋亡率和S期细胞的比例逐渐升高；Western Blot显示，随着丹酚酸乙浓度的增加，MCF-7细胞caspase-3蛋白表达水平逐渐升高。［结论］ 丹酚酸乙对MCF-7细胞具有明显的生长抑制和促凋亡作用，这种作用可能与上调caspase-3表达以及阻滞细胞于S期有关。 |
| 英文摘要: |
| Abstract: ［Purpose］ To observe the effect of salvianolic acid B on growth inhibition and apoptosis induction in breast cancer MCF-7 cells. ［Methods］ The effect of salvianolic acid B on inhibition of MCF-7 cell proliferation was detected by MTT assay，and the cellular morphologic apoptosis was observed by Hoechst33258 staining. Cell flow cytometry was utilized to observe the apoptosis rate and cell cycle of MCF-7 cells incubated with salvianolic acid B for 48h. Expression of caspase-3 was detected by Western Blot. ［Results］ Salvianolic acid B obviously inhibited the growth of MCF-7 cells in a time- and dose-dependent manner. Fluorescence staining indicated that the MCF-7 cells showed some obvious apoptosis characteristics such as，nuclear condensation，fragmentation，chromatin condensation and apoptotic body formation，when the cells cultured with salvianolic acid B (0.7mg/ml) for 24，48 and 72h. FCM assay indicated that apoptosis rate and percentages of MCF-7 cells in S phase gradually increased after incubated with salvianolic acid B for 48h. Western Blot showed that the expression level of caspase-3 protein gradually rose with the increase of salvianolic acid B concentration. ［Conclusion］ Salvianolic acid B can inhibit cell growth and induce cell apoptosis，which might relate to S stage arrested and upregalation of caspase-3 expression in MCF-7 cells. |
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