| 问 静,何 侠,赵洪瑜.高能X线和人参皂甙Rb1对人脑胶质瘤细胞SHG-44的影响[J].肿瘤学杂志,2012,18(4):266-269. |
| 高能X线和人参皂甙Rb1对人脑胶质瘤细胞SHG-44的影响 |
| Effects of High Energy X-ray and Ginsenoside Rb1 on Human Glioma SHG-44 Cell |
| 投稿时间：2011-11-07 |
| DOI： |
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| 中文关键词: 高能X线 人参皂甙Rb1 胶质细胞瘤 基质金属蛋白酶 |
| 英文关键词:high energy X-ray ginsenoside Rb1 glioma MMPs |
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| 摘要点击次数: 1777 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨高能X线和人参皂甙Rb1对人脑胶质瘤细胞株SHG-44的影响。［方法］ 应用MTT法检测细胞增殖情况；流式细胞仪检测细胞周期的变化；RT-PCR法检测MMP-２、MMP-９ mRNA的表达水平。［结果］ 高能X线照射后SHG-44细胞增殖受到抑制，且随照射剂量加大抑制作用增强。当加入人参皂甙Rb1后，细胞增殖抑制更加明显；高能X线照射后SHG-44细胞表现为G2/M期阻滞，而人参皂甙Rb1可缓解G2/M期阻滞现象；辐射促进MMP-２、MMP-９的表达，且随照射剂量的增加表达量增加，而人参皂甙Rb1抑制其表达，与药物浓度呈反比。［结论］ 人参皂甙Rb1与高能X线协同抑制SHG-44的增殖，辐射后肿瘤细胞侵袭能力增强，而人参皂甙Rb1使照射后肿瘤细胞侵袭能力下降。 |
| 英文摘要: |
| Abstract: ［Purpose］ To investigate the effects of high energy X-ray and ginsenoside Rb1 on human glioma SHG-44 cell line. ［Methods］ MTT method was applied to detect the cell proliferation conditions. Flow cytometry was applied to observe the change of cell cycle. The expressions of MMP-２ and MMP-９ mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR). ［Results］ The SHG-44 cell proliferation was inhibited after 12h of radiation，which boosted with the increasing of radiation dose. The inhibition of proliferation was more obvious when adding ginsenoside Rb1. The SHG-44 cell showed that the G2/M phase arrested after radiation，and the delay of G2/M phase alleviated when adding ginsenoside Rb1. The expressions of MMP-２ and MMP-９ in SHG-44 cell increased along with the increasing of X-ray dose. When ginsenoside Rb1 was added to the cell culture，the expressions of MMP-２ and MMP-９ were inhibited，which decreased along with increasing of the dose of ginsenoside Rb1.［Conclusion］ Ginsenoside Rb1 and high energy X-ray radiation show synergistic inhibition of cell proliferation. After radiation，the ability of tumor invasion and metastasis increases，while ginsenoside Rb1 can decrease the ability of invasion and metastasis by the down-regu-lation of MMPs mRNA expression. |
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