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| 多模态显影载药微球应用于肝肿瘤经动脉化疗栓塞的实验研究 |
| Experimental study on the application of multimodal imaging drug-loaded microspheres in transcatheter arterial chemoembolization for liver tumors |
| 投稿时间：2025-12-04 修订日期：2026-02-23 |
| DOI： |
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| 中文关键词: 纳米组装载药微球 动脉栓塞术 兔 肝肿瘤 实验性肿瘤 阿霉素 |
| 英文关键词:nano-assembled microspheres arterialembolization rabbit liver cancer experimental tumor doxorubicin |
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| 摘要点击次数: 72 |
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| 中文摘要: |
| [目的]在动物肝癌模型中验证多模态显影纳米组装载药微球(Nano assembled microspheres, NAMs)的X射线/CT/MR显影功能和经动脉化疗栓塞效果。[方法]基于前期研究,制备多模态显影纳米组装载药微球,并将其应用于兔肝肿瘤化疗栓塞研究。利用VX2瘤株构建兔肝癌模型,使用NAMs在DSA引导下行肝动脉栓塞术。通过术前术后CT和MRI扫描及术中数字减影血管造影(digital subtraction angiography,DSA),研究纳米组装微球多模态显影功能。通过DSA、CT扫描和H E、Ki67、TUNEL病理检查,研究负载阿霉素的纳米组装微球动脉化疗栓塞效果；该研究共成功纳入45只兔子,实验分组:对照组15只(推注生理盐水)、空白微球组15只(推注空白微球)、阿霉素(DOX doxorubicin)载药微球组15只(推注加载阿霉素的NAMs Doxorubicin-Nano assembled microspheres DOX-NAMs)。[结果]微导丝段定位针在兔子肝内行CT扫描伪影影响范围较小,可作为肝内肿瘤标记物；NAMs在兔VX2肝肿瘤血管和肿瘤内实现X射线/CT/MR多模态下显影；利用DOX-NAMs经肝动脉栓塞,术后CT、MR扫描可见微球显影。病理结果显示相对于空白微球和对照组,DOX-NAMs栓塞组肝内肿瘤坏死程度更高,化疗栓塞效果更好。[结论]多模态纳米组装载药微球这一新型载药栓塞微球在动物体内具备良好的多模态CT/MRI可视化显影能力,及优异的动脉化疗栓塞效果和抗肿瘤效果,在介入栓塞应用中具有极大的优势和潜力。 |
| 英文摘要: |
| [Objective]: To verify the X-ray/CT/MR imaging function and transarterial chemoembolization effect of multimodal imaging nano-assembled drug-loaded microspheres (NAMs) in an animal liver cancer model. [Methods]: Based on previous research, multimodal imaging nano-assembled drug-loaded microspheres were prepared and applied in the study of transarterial chemoembolization for rabbit liver tumors. A rabbit liver cancer model was established using the VX2 tumor strain, and NAMs were used for hepatic artery embolization under DSA guidance. The multimodal imaging function of nano-assembled microspheres was studied through preoperative and postoperative CT and MRI scans and intraoperative digital subtraction angiography (DSA). The transarterial chemoembolization effect of doxorubicin-loaded nano-assembled microspheres was investigated by DSA, CT scans, and H E, Ki67, and TUNEL pathological examinations. A total of 45 rabbits were successfully included in this study, and the experimental groups were as follows: 15 rabbits in the control group (injected with normal saline), 15 rabbits in the blank microsphere group (injected with blank microspheres), and 15 rabbits in the doxorubicin (DOX) loaded nano-assembled microsphere group (injected with DOX-NAMs). [Results]: The CT scan artifacts caused by the microcatheter positioning needle in the rabbit liver were relatively small, and it could be used as a marker for liver tumors. NAMs achieved multimodal X-ray/CT/MR imaging in the blood vessels and tumors of rabbit VX2 liver tumors. After transarterial embolization with DOX-NAMs, microsphere imaging was visible in postoperative CT and MR scans. Pathological results showed that the degree of tumor necrosis in the DOX-NAMs embolization group was higher than that in the blank microsphere and control groups, and the chemoembolization effect was better. [Conclusion]:The novel drug-loaded embolization microsphere, multimodal nano-assembled drug-loaded microspheres, has good multimodal CT/MRI visualization imaging ability, excellent transarterial chemoembolization effect, and anti-tumor effect in animal bodies, and has great advantages and potential in interventional embolization applications. |
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