|
APLN在食管鳞癌中表达的临床意义及其对食管鳞癌恶性表型侵袭和迁移的影响 |
Clinical significance of APLN expression in oesophageal squamous carcinoma and its effect on invasion and migration of the malignant phenotype of oesophageal squamous carcinoma |
投稿时间:2024-10-24 修订日期:2025-01-03 |
DOI: |
|
|
中文关键词: 食管鳞癌 APLN 预后 侵袭 转移 |
英文关键词:esophageal squamous cell carcinoma APLN prognosis invasion metastasis |
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目) |
|
摘要点击次数: 78 |
全文下载次数: 0 |
中文摘要: |
目的:分析食管鳞癌中APLN的表达水平,并探讨其临床意义,进一步研究APLN对食管鳞癌恶性表型侵袭和迁移的影响。方法:利用公共数据库,分析APLN在食管鳞癌中的表达、与患者临床病理资料及生存预后的影响。选取2016年1月-2017年12月期间我院收治的163例ESCC患者作为研究对象,收集所有患者的临床资料、随访相关资料,同时将病理标本制作成组织芯片。采用免疫组织化学染色对APLN的表达及其与患者病理和预后进行验证分析。进一步在食管鳞癌细胞和动物模型中分析APLN对ESCC的生物行为学影响。结果:公共数据库中分析显示APLN在ESCC中的表达水平显著高于癌旁组织(P<0.05),且高表达患者预后较差。临床样本RT-PCR和组织芯片免疫组化验证结果均显示APLN在ESCC中的表达水平显著高于癌旁组织(P<0.05),且免疫组化结果表明,ESCC患者肿瘤组织中APLN表达与N分期及神经脉管侵犯显著相关。单因素及多因素Logistic回归分析显示较高的N分期及APLN的高表达均与ESCC患者较差的预后相关。在ESCC细胞株中,Western Blot和RT-PCR技术分析结果显示APLN在KYSE150合KYSE-30细胞中的表达水平现在高于人正常食管上皮细胞(P<0.05)。在KYSE-30细胞中,抑制APLN的表达可以显著降低细胞的侵袭和迁移能力,过表达APLN的表达可以显著增强细胞的侵袭和迁移能力。在动物模型中,敲低APLN可以减缓肿瘤生长,而过表达APLN则会加快肿瘤的生长。结论:在食管鳞癌中,APLN的表达显著高于正常组织,与患者N分期及神经脉管侵犯显著相关,且显著影响患者的预后。APLN能够促进ESCC细胞的恶性表型。 |
英文摘要: |
Objective:To analyze the expression level of APLN in ESCC and explore its clinical significance to further investigate the effect of APLN on invasion and migration of ESCC. Methods: Using public databases, analyze the expression of APLN in esophageal squamous cell carcinoma and its impact on patient clinical pathological data and survival prognosis. 163 ESCC patients admitted to our hospital from January 2016 to December 2017 were selected as the research subjects. Clinical and follow-up data of all patients were collected, and pathological specimens were made into tissue chips. Immunohistochemical staining was used to verify and analyze the expression of APLN and its correlation with patient pathology and prognosis. Further analysis the biobehavioral effects of APLN on ESCC in esophageal squamous carcinoma cells and animal models. Results: Analysis in public databases shows that the expression level of APLN in ESCC is significantly higher than that in adjacent tissues (P<0.05), and patients with high expression have poor prognosis. The clinical sample RT-PCR and tissue chip immunohistochemistry validation results showed that the expression level of APLN in ESCC was significantly higher than that in adjacent tissues (P<0.05), and immunohistochemistry results showed that the expression of APLN in ESCC patients" tumor tissues was significantly correlated with N staging and neurovascular invasion. Univariate and multivariate logistic regression analysis showed that higher N staging and high expression of APLN were associated with poorer prognosis in ESCC patients.In the ESCC cell lines, Western Blot and RT-PCR technical analysis showed that the expression level of APLN in KYSE150 and KYSE-30 cells is now higher than in human normal esophageal epithelial cells (P<0.05). In KYSE-30 cells, inhibition of APLN expression significantly reduced cell invasion and migration capacity, and overexpression of APLN expression significantly enhanced cell invasion and migration capacity. In animal models, knockdown of APLN slows tumor growth, while overexpression of APLN accelerates tumor growth. Conclusions: In ESCC, APLN expression is significantly higher than normal tissue, which is significantly associated with patient N stage and neural vascular invasion, and significantly affects the prognosis of patients. APLN is able to promote the malignant phenotype of ESCC cells. |
在线阅读
查看/发表评论 下载PDF阅读器 |
|
|
|