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| Syncytin-1和cripto-1在NSCLC患者胸腔积液外泌体中表达及意义 |
| Expression and clinical significance of syncytin-1 and cripto-1 in exosomes of pleural effusion in non-small cell lung cancer |
| 投稿时间：2024-05-23 修订日期：2024-07-14 |
| DOI： |
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| 中文关键词: 非小细胞肺癌 外泌体 Syncytin-1 Cripto-1 呼吸道肿瘤标志物 |
| 英文关键词:non-small cell lung cancer Exosome Syncytin-1 Cripto-1 Respiratory tumor markers |
| 基金项目:山东省自然科学基金（ZR2020MH321） |
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| 摘要点击次数: 57 |
| 全文下载次数: 0 |
| 中文摘要: |
| [目的] 探讨合胞素1（syncytin-1）和畸胎癌衍生生长因子-1（cripto-1）在非小细胞肺癌（non-small cell lung cancer, NSCLC）患者胸腔积液外泌体中的表达情况及其临床意义。[方法] 对41例首诊为NSCLC的患者和41例肺炎患者的胸腔积液进行外泌体的提取、鉴定、RNA提取及syncytin-1、cripto-1表达的检测。 通过蛋白印迹法 （Western blotting, WB）检测外泌体蛋白表达，并利用酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay, ELISA）检测外泌体内蛋白含量。分析syncytin-1和cripto-1在NSCLC患者胸腔积液外泌体中的表达量与临床病理特征的相关性，并基于统计学手段评估其对NSCLC的诊断效能。[结果] 与肺炎患者相比，NSCLC患者的胸腔积液外泌体中Syncytin-1和cripto-1的表达量升高且有统计学意义（P<0.0001）。WB检测显示外泌体表达其特异性蛋白，ELISA检测结果表明syncytin-1和cripto-1在NSCLC患者组和肺炎患者组之间存在统计学差异（P<0.0001）。此外，syncytin-1和cripto-1的测定值对NSCLC辅助诊断具有82.9%和75.6%的灵敏度、75.6%和80.5%的特异度，对应的曲线下面积（area under curve, AUC）分别为0.847和0.849。相比之下，它们的AUC值高于肿瘤标志物神经元特异性烯醇化酶（neuron-specific enolase，NSE）和胃泌素释放肽前体（progastrin-releasing peptide, ProGRP）（AUC分别为0.762和0.716），但低于癌胚抗原（carcinoembryonic antigen，CEA）（AUC=0.931），差异具有统计学意义（P<0.001）。[结论] 胸腔积液外泌体中syncytin-1、cripto-1的检测对于诊断NSCLC具有较高的灵敏度和特异度，可作为辅助检测NSCLC的新型分子标志物。 |
| 英文摘要: |
| [Objective] To investigate the expression levels and clinical significance of syncytin-1 and cripto-1 in pleural effusion of patients diagnosed with non-small cell lung cancer (NSCLC). [Methods] The expression of syncytin-1 and cripto-1 in the pleural effusion of 41 NSCLC and 41 pneumonia patients were detected by extraction and identification of exosomes and RNA. Westernblotting was used to detect exosomal protein expression, and Enzyme-Linked Immunosorbent Assay (ELISA) was ultilized to mesure the content of exoprotein. The association between expression levels of syncytin-1 and cripto-1 in exosomes in pleural effusion of NSCLCpatients and clinicopathological features were analyzed, and the diagnostic efficacy of pleural effusion exosomes syncytin-1 and cripto-1 in NSCLC was statistically evaluated. [Results] Compared with patients diagnosed with pneumonia, the expression levels of syncytin-1 and cripto-1 in exosomes extracted from pleural effusion of NSCLC patients showed a significantly increase (P<0.0001). Western blotting analysis was used to detect exosomes expressing their specific proteins. Statistically significant differences were observed in the exosome ELISA detection of syncytin-1 and cripto-1 between NSCLC and pneumonia patients (P<0.0001). The detection of syncytin-1 and cripto-1 for the auxiliary diagnosis of NSCLC showed a sensitivity of 82.9% and 75.6%, a specificity of 75.6% and 80.5%, and the corresponding area under the curve (AUC) values were 0.847 and 0.849, respectively. In comparison, their AUC values were higher than those of traditional respiratory tumor markers neuron-specific enolase (NSE) and progastrin-releasing peptide (ProGRP) (AUCs of 0.762 and 0.716, respectively), but lower than that of carcinoembryonic antigen (CEA) (AUC=0.931) with statistical difference (P<0.001). [Conclusion] The assessment of exosomes syncytin-1 and cripto-1 in exosomes from pleural effusion exhibited high sensitivity and specificity in NSCLC diagnosis, offering promise as a novel molecular marker for assisting the detection of NSCLC. |
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