| 张光军,郑起,袁周,王志刚,黄新余.槐耳清膏体外诱导人肝癌细胞MHCC97H凋亡[J].肿瘤学杂志,2010,16(4):. |
| 槐耳清膏体外诱导人肝癌细胞MHCC97H凋亡 |
| Trametes Robiniophila Murr Induced Apoptosis of Hepatocellular Carcinoma Cell Line MHCC97H |
| 中文关键词 |
| DOI： |
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| 中文关键词: 槐耳清膏 诱导 肝癌细胞 凋亡 |
| 英文关键词:Trametes robiniophila Murr induce hepatocellular carcinoma cells apoptosis |
| 基金项目: |
| 张光军 郑起 袁周 王志刚 黄新余 |
| 上海交通大学医学院附属第六人民医院; |
| 摘要点击次数: 2446 |
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| 中文摘要: |
| [目的]研究槐耳清膏对人肝癌细胞MHCC97H凋亡的作用及其机制。[方法]不同浓度槐耳清膏及5-Fu分别与人肝癌细胞MHCC97H共同培养,采用CCK-8法、FCM及荧光显微镜检测细胞增殖与凋亡变化,细胞免疫组化检测Bax、Bcl-2及p53基因的表达。[结果]槐耳清膏在体外可抑制MHCC97H细胞的增殖,诱导MHCC97H细胞产生早期凋亡,且作用具有剂量和时间依赖性。荧光显微镜下细胞呈典型的早期凋亡形态学改变。细胞免疫组化可见Bax及p53蛋白表达增强,而Bcl-2蛋白表达减少。[结论]槐耳清膏能明显诱导人肝癌细胞MHCC97H凋亡,其机制可能与改变凋亡相关基因Bax、Bcl-2、p53的表达有关。 |
| 英文摘要: |
| [Purpose] To investigate the effect and mechanism of Trametes robiniophila Murr induced apoptosis of human hepatocellular carcinoma cell line MHCC97H. [Methods] MHCC97H cells were incubated with different dose of Trametes robiniophila Murr or 5-Fu. Cell growth and apoptosis were assessed by CCK-8, flow cytometr (FCM) and fluorescence microscopy. The change of Bax, Bcl-2 and p53 expression were detected by cell immunohistochemistry. [Results] The growth of MHCC97H cells was inhibited and the apoptosis was in... |
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