| 陈雪琴,杨邵瑜,潘月龙,张玮,王建,刘继洪.热放疗对人肺腺癌耐药细胞株A549/DDP凋亡的影响[J].肿瘤学杂志,2010,16(4):. |
| 热放疗对人肺腺癌耐药细胞株A549/DDP凋亡的影响 |
| Effect of Radiohyperthermia on Apoptosis in Multidrug-resistant Human Lung Adenocarcinoma Cell Line A549/DDP |
| 中文关键词 |
| DOI： |
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| 中文关键词: 热疗 放射疗法 肺肿瘤 凋亡 |
| 英文关键词:hyperthermia radiotherapy lung neoplasms apoptosis |
| 基金项目:杭州市医药卫生科技计划重点项目(2007Z002) |
| 陈雪琴 杨邵瑜 潘月龙 张玮 王建 刘继洪 |
| 杭州市第一人民医院; |
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| 中文摘要: |
| [目的]观察热放疗对人肺腺癌耐药细胞株A549/DDP凋亡作用的影响。[方法]MTT法细胞毒性实验和克隆形成实验检测热放疗对A549/DDP细胞抑制作用和克隆形成的影响;TUNEL法检测热放疗对细胞凋亡作用的影响;透射电镜观察各组细胞形态学改变。[结果]细胞毒性实验和克隆形成实验显示热放疗对A549/DDP细胞生长有明显抑制作用(P<0.01);TUNEL法观察到热放疗有诱导A549/DDP细胞凋亡作用;电子显微镜观察到热放疗后A549/DDP出现细胞核固缩、变形,染色质浓聚、边集等凋亡现象。[结论]热放疗能诱导A549/DDP细胞凋亡。 |
| 英文摘要: |
| [Purpose] To investigate the effect of radiohyperthermia on apoptosis in multidrug-resistant human lung adenocarcinoma cell line A549/DDP. [Methods] Effect of radiohyperthermia on A549/DDP cell inhibition ratio and clone formation rate were detected by MTT assay and clone formation experiment. A549/DDP cell apoptosis phenomenon was detected by TUNEL assay. Morphological changes of cells were observed by transmission electron microscopy. [Results] MTT assay and clone formation experiment showed that radiohyp... |
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