| 杨立涛,李伟,郑雪咏.CO_2气腹对结肠癌SW480细胞株生长周期及CD44表达的影响[J].肿瘤学杂志,2010,16(2):. |
| CO_2气腹对结肠癌SW480细胞株生长周期及CD44表达的影响 |
| Impact of CO_2 Pneumoperitoneum to the Cytocycle and CD44 Expression in SW480 Colon Cancer Cell |
| 中文关键词 |
| DOI：10.11735/j.issn.1671-170X.2010.2.025 |
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| 中文关键词: 结肠肿瘤 CO2气腹 细胞周期 CD44 |
| 英文关键词:colon neoplasms CO2 pneumoperitoneum cytocyle CD44 |
| 基金项目:浙江省教育厅科研项目(20070063) |
| 杨立涛 李伟 郑雪咏 |
| 浙江省肿瘤医院;浙江大学医学院附属邵逸夫医院; |
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| 中文摘要: |
| [目的]探讨CO2气腹对体外培养的结肠癌细胞生长增殖的影响。[方法]采用CO2气体建立模拟气腹条件,对照组不作气腹处理。CO2组、Ar组处理结肠癌SW480细胞株气压均为15mmHg维持30min,观察其细胞生长周期变化及细胞表面黏附分子CD44表达情况。[结果]CO2气腹处理SW480结肠癌细胞24h后S期细胞比例无明显变化,与对照组比较无显著性差异;48h后S期细胞比例降至7.2%,显著性低于对照组的37.2%;72h后S期比例回升至25.7%,与对照组相比无显著性差异(25.7%vs31.1%,P>0.05)。而CO2组CD44表达在24h后降低至1.2%,低于对照组的13.4%(P<0.01);48h和72hCD44表达均高于对照组(26.4%vs13.4%,P<0.01;39.7%vs13.4%,P<0.01)。[结论]CO2气腹对体外培养的结肠癌SW480细胞的生长有短暂可逆的抑制作用。 |
| 英文摘要: |
| [Purpose] To investigate the impact of CO2 pneumoperitoneum on potential of cell growth and proliferation in colon cancer cells.[Methods] The SW480 colon cancer cells were exposed to CO2 gases for 30 minutes with ambient pressure 15mmHg.The cell phases and CD44 expression were determined.[Results] Compared to control group,the cell proportion of S phase at 24h after CO2 pneumoperitoneum was not obviously changed;at 48h,it decreased to 7.2% at 48h with significant difference(7.2% vs 37.2%,P<0.01),it increase... |
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