| 张华,李苏宜,崔玖洁,赵婷.刺梨和金荞麦提取物体外干预人食管癌细胞株CaEs-17增殖和凋亡[J].肿瘤学杂志,2010,16(1):. |
| 刺梨和金荞麦提取物体外干预人食管癌细胞株CaEs-17增殖和凋亡 |
| Effects of Extract from Rosa Roxburghir Tratt or Extract of Fagopyrum Cymosum Meissn on Cell Proliferation and Apoptosis in Human Esophageal Carcinoma CaEs-17 Cell Line |
| 中文关键词 |
| DOI： |
|
 |
| 中文关键词: 刺梨 CL 金荞麦 FR 食管肿瘤 凋亡 增殖 |
| 英文关键词:roxburghir rose CL fagopyrum cymosum FR esophageal neoplasms apoptosis proliferation |
| 基金项目: |
| 张华 李苏宜 崔玖洁 赵婷 |
| 东南大学临床医学院;东南大学附属中大医院; |
| 摘要点击次数: 2063 |
| 全文下载次数: 0 |
| 中文摘要: |
| [目的]探讨体外环境下,刺梨提取物(CL)联合金荞麦提取物(FR)对人食管癌细胞株CaEs-17增殖及凋亡的影响。[方法]MTT法检测CaEs-17细胞的增殖抑制率;流式细胞术检测CaEs-17细胞凋亡率;RT-PCR和Western blot法分析Ki-67、Bax及Bcl-2表达的变化。[结果]CL、FR单独应用及两者联合均可明显抑制CaEs-17生长,且呈剂量依赖性;IC30浓度的CL、FR和1/2(IC30CL+IC30FR)对CaEs-17的抑制率分别为26.87%±0.29%、25.20%±0.42%和39.58%±1.07%,流式细胞术结果表明对照组、CL组、FR组、联合组细胞早期凋亡比例依次增加,两单药组分别与联合组比较差异均有统计学意义(P<0.05);与单药组比较,联合组Ki-67、Bcl-2 mRNA/蛋白表达率有显著降低,而Bax mRNA/蛋白基因表达显著增高。[结论]CL、FR均可抑制人食管癌细胞株CaEs-17增殖和诱导其凋亡,两者在体外有协同作用。 |
| 英文摘要: |
| [Purpose] To investigate the effect of CL plus FR on cell proliferation and apoptosis in human esophageal carcinoma CaEs-17 cell line in vitro.[Methods] The cells proliferation inhibition were detected by MTT assay.Cell apoptosis rate was detected by flow cytometry.The changes of Ki-67,Bax and Bcl-2 expression were quantified by RT-PCR and Western blot.[Results] The CaEs-17 cell proliferation was significantly inhibited by CL or FR alone and CL plus FR,with a dose-dependent manner.The inhibitory rate of CL ... |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|