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| 胰腺癌患者血浆LINC01268及miR-582-5p水平的表达及临床意义 |
| Expression and clinical significance of plasma LINC01268 and miR-582-5p in patients with pancreatic cancer |
| 投稿时间：2024-07-22 修订日期：2024-10-07 |
| DOI： |
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| 中文关键词: 胰腺癌 LINC01268 miR-582-5p 临床意义 |
| 英文关键词:Pancreatic cancer LINC01268 miR-582-5p Clinical significance |
| 基金项目:海南省医药卫生科研(21A200074) |
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| 中文摘要: |
| 胰腺癌患者LINC01268表达升高,miR-582-5p表达降低,且与肿瘤分期和淋巴结转移有关,LINC01268、miR-582-5p及CA199三项联合检测对早期胰腺癌具有较好的诊断价值。 |
| 英文摘要: |
| 【】? Objective? To investigate the expression of LINC01268 and miR-582-5p in pancreatic cancer and their diagnostic value in early pancreatic cancer. Methods? 107 patients with pancreatic cancer (pancreatic cancer group), 80 patients with pancreatitis (benign group) and 50 patients with normal physical examination (control group) admitted to the hospital from January 2022 to March 2024 were selected as research objects. 107 patients with pancreatic cancer were divided into 31 patients with early pancreatic cancer and 76 patients with intermediate and advanced pancreatic cancer. Fluorescence quantitative PCR technology was used to detect the levels of LINC01268 and miR-582-5p, and chemiluminescence method was used to measure the level of carbohydrate antigen 199 (CA199). Multivariate logistic regression analysis was used to analyze the risk factors affecting the occurrence of middle and advanced pancreatic cancer, and ROC curve was drawn to analyze the diagnostic value of LINC01268, miR-582-5p and CA199 levels in early pancreatic cancer. Pearson correlation was used to analyze the correlation between plasma LINC01268, miR-582-5p levels and CA199 in pancreatic cancer patients. Results? The expression level of LINC01268 (2.68±1.04 vs 1.02±0.35) in pancreatic cancer tissues was significantly higher than that in paracancerous tissues, while the expression level of miR-582-5p (0.51±0.13 vs 1.26±0.43) was significantly lower than that in paracancerous tissues(P<0.001). The levels of plasma LINC01268 and CA199 in pancreatic cancer group were significantly higher than those in benign group and control group, while the plasma level of miR-582-5p in pancreatic cancer group was significantly lower than those in benign group and control group (P<0.001). The levels of plasma LINC01268 and CA199 were significantly increased in the middle and advanced pancreatic cancer group, while the levels of plasma miR-582-5p were significantly reduced (P<0.001). The levels of plasma LINC01268 and CA199 were significantly increased in the lymph node metastasis group, while the levels of plasma miR-582-5p were significantly reduced (P<0.001). Multivariate logistic regression analysis showed that increased levels of LINC01268 (OR=4.105, 95% CI: 2.983-12.864) and CA199 (OR=2.790, 95% CI: 2.307-10.116), and decreased levels of miR-582-5p (OR=2.476, 95% CI: 1.915-9.521) were risk factors for advanced pancreatic cancer (all P<0.05). ROC curve analysis showed that the combination of LINC01268≥2.16, miR-582-5p≤0.57 and CA199≥64.82 U/ml had the maximum area under the curve (AUC) 0.925 (95% CI: 0.862-0.990) in the diagnosis of early pancreatic cancer, and its accuracy was 87.6%. Correlation analysis showed that the plasma LINC01268 level in pancreatic cancer patients was positively correlated with CA199 (r=0.825, P<0.001), and the plasma miR-582-5p level was negatively correlated with CA199 (r= - 0.716, P<0.001). Conclusion? In patients with pancreatic cancer, the expression of LINC01268 is increased, and the expression of miR-582-5p is decreased, which is related to tumor stage and lymph node metastasis. The combined detection of LINC01268, miR-582-5p and CA199 has a good diagnostic value for early pancreatic cancer. |
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