| [Objective] To explore the effect and mechanism of microRNA-183 (miR-183) on the bladder cancer (BCa) disease progression. [Methods] Human bladder cancer cell line T24 was cultured in vitro. The exprssions of miR-183 and leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) were inhibited respectively/combined. LRIG1 mRNA expression was mesured by qPCR. Western blot (WB) was used to detect the protein expressions of LRIGI and epidermal growth factor receptor (EGFR). CCK-8, scratch, Transwell assay and flow cytometry were used to detect cell proliferation, cell migration, cell invasion and apoptosis, respectively. [Results] Compared with NC, LRIG1 protein expression significantly increased but EGFR protein expression significantly decreased in the miR-183 inhibitor group cells (P<0.05), while LRIG1 protein expression significantly decreased but EGFR protein expression significantly increased in the shLRIG1 group cells (P<0.05); the shLRIG1+miR-183 inhibitor reversed the aforementioned changes caused by the knockdown of single factor. In addition, the cell proliferation, migration, and invasion decreased but the apoptosis increased in the miR-183 inhibitor group (P<0.05), while the cell proliferation, migration, and invasion increased but the apoptosis decreased in the shLRIG1 group (P<0.05); there were no significant differences in the biological functions between the NC group and the shLRIG1+miR-183 inhibitor group. [Conclusions] MiR-183 may regulate EGFR expression by targeting LRIG1 and then affect the biological functions in bladder cancer cell T24. |
