| 张孝艳,赵先兰,周 艳.MiRNA-210通过PI3/AKT信号通路上调E2F3促进卵巢癌SKOV3细胞增殖作用[J].中国肿瘤,2019,28(10):792-796. |
| MiRNA-210通过PI3/AKT信号通路上调E2F3促进卵巢癌SKOV3细胞增殖作用 |
| MiRNA-210 Promotes Proliferation of Ovarian Cancer SKOV3 Cells by Up-regulating E2F3 via PI3/AKT Signaling Pathway |
| 投稿时间：2018-09-19 |
| DOI：10.11735/j.issn.1004-0242.2019.10.A013 |
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| 中文关键词: miRNA-210 卵巢肿瘤 SKOV3 PI3/AKT |
| 英文关键词:miRNA-210 ovarian neoplasms SKOV3 PI3/AKT |
| 基金项目:河南省科技攻关计划项目（132102310145） |
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| 中文摘要: |
| 摘 要：［目的］ 研究miRNA-210是否通过PI3/AKT信号通路上调E2F3促进卵巢癌SKOV3细胞的增殖作用。［方法］ 收集24例卵巢癌患者的临床组织。QRT-PCR分别检测肿瘤组织和癌旁组织中miRNA-210的表达；Western blot检测肿瘤组织和癌旁组织中AKT、P-AKT、E2F3表达；CCK-8检测三组细胞的增殖能力；平板克隆形成实验检测三组细胞的克隆形成能力；裸鼠皮下荷瘤模型检测三组细胞的成瘤能力。［结果］ 肿瘤组织中miRNA-210的表达高于癌旁组织；肿瘤组织中P-AKT 和E2F3的表达高于癌旁组织；转染结束后，三组细胞中过表达载体miRNA-210转染SKOV3细胞中miRNA-210表达明显高于对照组和空白组，而对照组和空白组之间无差异；CCK-8检测结果显示miRNA-210过表达后促进了SKOV3细胞增殖；平板克隆形成实验结果显示miRNA-210过表达后SKOV3细胞克隆形成能力明显增强；裸鼠皮下荷瘤模型结果显示miRNA-210过表达后SKOV3细胞皮下成瘤能力明显强于对照组和空白组；Western blot结果显示miRNA-210过表达后P-AKT和E2F3表达增加，但是miRNA-210过表达SKOV3细胞系中加入1μmol/L Wortmannin后E2F3表达随之下调。［结论］ MiRNA-210通过PI3/AKT信号通路上调E2F3，从而促进卵巢癌SKOV3细胞增殖作用。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of miRNA-210 on proliferation of ovarian cancer SKOV3 cells and its molecular mechanism. ［Methods］ The tumor tissue samples of 24 patients with ovarian cancer were collected. QRT-PCR was used to detect the expression of miRNA-210 in tumor tissues and adjacent tissues. Western blot was used to detect the expression of AKT，P-AKT and E2F3 in tumor tissues and pericancerous tissues. Ovarian cancer SLOV3 cells were transfected with recombinant miRNA-210 vector(miRNA-210 group) and blank plasmid(blank group)，respectively. The clone formation ability of untransfected SLOV3 cells(control group)，miRNA-210 group and blank group was detected by plate clone formation assay；the proliferation of three groups of cells was detected by CCK-8. The SLOV3 cells of control group，miRNA group and blank group were inoculated subcutaneously in nude mice and the tumorigenic ability was examined.［Results］ The expression of miRNA-210 in tumor tissues was higher than that in adjacent tissues. The expression of P-AKT and E2F3 in tumor tissues was higher than that in adjacent tissues. The expression of miRNA-210 in SKOV3 cells of miRNA-210 group was significantly higher than that in the control group and the blank group，but there was no difference between the control group and the blank group. The CCK-8 test showed that the proliferation of SKOV3 cells in miRNA-210 group was promoted. Plate clone formation assay showed that the cloning ability of SKOV3 cells was significantly enhanced after transfected with miRNA-210. The tumorigenic ability of SKOV3 cells in miRNA-210 group was significantly stronger than that of other two groups. Western blot analysis showed that the expression of P-AKT and E2F3 in SKOV3 cells was increased after transfection of miRNA-210，but the expression of E2F3 was reduced after treated with 1μmol/L Wortmannin. ［Conclusion］ MicroRNA-210 can promote the proliferation of ovarian cancer SKOV3 cells，which is associated with the up-regulation of E2F3 via PI3/AKT signaling pathway. |
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