| 郭旭东,邵 毅,沈 艺.miR-15家族基因多态性及表达与食管鳞癌发生的研究[J].中国肿瘤,2019,28(6):470-475. |
| miR-15家族基因多态性及表达与食管鳞癌发生的研究 |
| Association Between Polymorphisms of miR-15 Family and the Risk of Esophageal Squamous Cell Carcinoma |
| 投稿时间：2019-03-26 |
| DOI：10.11735/j.issn.1004-0242.2019.06.A013 |
|
 |
| 中文关键词: 食管鳞癌 微小RNA miR-15家族 单核苷酸多态性 |
| 英文关键词:esophageal squamous cell carcinoma microRNA miR-15 family single nucleotide polymorphism |
| 基金项目:北京市自然科学基金(7132023) ；国家自然科学基金(81874277、 81473056、81573224)；北京市教委科研计划一般项目（KM201710025006） |
|
| 摘要点击次数: 1727 |
| 全文下载次数: 340 |
| 中文摘要: |
| 摘 要：［目的］ 通过病例对照设计来评估miR-15家族基因相关SNPs及其表达与食管鳞癌发生风险的关联。［方法］ 从2017年10月至2018年6月于河南省林州市肿瘤医院就诊患者中，选取食管癌新发患者43例为病例组，选取同期在当地医院参加食管癌早诊早治筛查项目的59名健康人群为对照组。对miR-15家族（miR-15a、miR-15b、 miR-16、miR-195和miR-497）中miRNAs基因上的10个单核苷酸多态性（single nucleotide polymorphisms，SNPs）位点（rs1022960、rs10936201、rs11078662、rs14309、rs1451761、rs2066557、rs2740545、rs412999、rs7998223、rs9535416）与食管鳞癌发生风险进行关联研究。同时，对miR-16-5p在食管癌患者血清中的表达差异性进行测定。采用χ2检验比较各SNPs位点基因型在病例组和对照组的分布，用秩和检验分析两组间miR-16-5p表达水平的差异；对单因素分析有统计学差异的变量采用多因素的Logistic回归进行校正。［结果］ 去除检测不合格的位点，最终纳入6个SNPs，各位点的基因型频率分布在病例组和对照组间差异均无统计学差异（P>0.05），食管鳞癌患者血清中miR-16-5p的表达量高于对照组(P＜0.001)。［结论］ miR-15家族中miR-16在食管鳞癌患者血清中高表达，但未发现候选的miRNAs多态位点与食管鳞癌发生风险相关。 |
| 英文摘要: |
| Abstract：［Purpose］ To evaluate the association between single nucleotide polymorphisms(SNPs) of miR-15 family and the risk of esophageal squamous cell carcinoma(ESCC). ［Methods］ Forty three ESCC patients admitted in Linzhou Cancer Hospital and 59 healthy subjects were recruited from October 2017 to June 2018. Genotypes were determined by MassArray based on allele-specific MALDI-TOF mass spectrometry. The expression level of miR-16-5p was detected by quantitative real-time PCR(qRT-PCR). The distribution of genotypes of each SNP was analyzed by χ2 test；Mann-Whitney U test was used to compare the expression change of miR-16-5p；the variables with statistical differences in univariate analysis were adjusted by multivariate logistic regression. ［Results］ After removing the unqualified SNPs，the genotype distribution of 6 SNPs included in the analysis was not associated with ESCC susceptibility(P>0.05). However，the expression level of the serum miR-16-5p in ESCC patients was significantly higher than that in healthy controls(P＜0.001). ［Conclusion］ The polymorphisms in miR-15 family may be not associated with the risk of ESCC，but the expression of serum miR-16 is significantly upregulated in ESCC patients. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |