| 张浩鹏,李国东,魏九峰.红树莓提取物抑制肝癌SMMC-7721细胞增殖的实验研究[J].中国肿瘤,2019,28(2):155-160. |
| 红树莓提取物抑制肝癌SMMC-7721细胞增殖的实验研究 |
| Effect of Red Raspberry Extract on Proliferation of Human Hepatocellular Carcinoma SMMC-7721 Cells |
| 投稿时间：2018-05-09 |
| DOI：10.11735/j.issn.1004-0242.2019.02.A015 |
|
 |
| 中文关键词: 红树莓提取物 肝肿瘤 细胞周期阻滞 |
| 英文关键词:red raspberry extract hepatocellular neoplasms cell cycle arrest |
| 基金项目:哈尔滨市科技创新人才研究专项资金项目（2017RAXXJ057）；哈尔滨医科大学研究生创新科研项目（YJSCX2017-49HYD） |
|
| 摘要点击次数: 2202 |
| 全文下载次数: 545 |
| 中文摘要: |
| 摘 要：［目的］ 探讨红树莓提取物抑制肝癌SMMC-7721细胞增殖的具体机制，为红树莓提取物预防并治疗肝癌提供理论依据。［方法］ 体外培养SMMC-7721细胞，应用CCK8方法及集落形成实验检测不同浓度的红树莓提取物对肝癌SMMC-7721细胞增殖活性的影响；通过流式细胞术检测SMMC-7721细胞经不同浓度的红树莓提取物作用72h后细胞周期的变化；应用Western blot方法检测红树莓提取物处理SMMC-7721细胞72h后细胞周期相关蛋白的表达变化，以及红树莓提取物对肝癌细胞AKT通路的影响。［结果］ 红树莓提取物能够抑制SMMC-7721细胞的增殖活性；不同浓度红树莓提取物处理SMMC-7721细胞72h后，与对照组相比，随着浓度的增高，G0/G1期细胞数比例降低（P＜0.05），S期细胞数比例升高（P＜0.05）；红树莓提取物能够降低周期相关蛋白cyclinA及CDK2的表达（P＜0.01）；同时使P-AKT(Ser473)表达下降（P＜0.01）。［结论］ 红树莓提取物能够抑制肝癌SMMC-7721细胞的增殖，这种抑制作用可能与AKT通路调控的cyclinA-CDK2介导的S期阻滞相关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of red raspberry extract on the proliferation of human hepatocellular carcinoma SMMC-7721 cells. ［Methods］ Hepatocellular carcinoma SMMC-7721 cells were treated with different concentrations of red raspberry extract for 72h，the cell proliferation was detected by CCK8 and colony formation assay. The cell cycle of SMMC-7721 cells were detected by flow cytometry；the expression of proteins related to cell cycle and AKT pathway was detected by Western blot. ［Results］ Red raspberry extract inhibited the proliferation of SMMC-7721 cells. Compared with control group，the proportion of cells of G0/G1 phase in red raspberry extract-treatment groups was significantly decreased(P＜0.05) and that of S phase increased(P＜0.05). The expression of cyclinA and CDK2 as well as the expression of P-AKT (Ser473) in raspberry extract-treatment groups were decreased(P＜0.01). ［Conclusion］ Red raspberry extract can inhibit the proliferation of human hepatocellular carcinoma SMMC-7721 cells，and the inhibitory effect is associated with S-phase arrest mediated by the AKT pathway. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |