| 刘 浩,孙丽娜,卢瑗瑗.miR-302c在胃癌中表达及对胃癌细胞侵袭和迁移影响的机制研究[J].中国肿瘤,2019,28(2):150-154. |
| miR-302c在胃癌中表达及对胃癌细胞侵袭和迁移影响的机制研究 |
| MiR-302c Inhibits Migration and Invasion of Gastric Cancer Cells by Targeting CREB1 |
| 投稿时间：2018-10-10 |
| DOI：10.11735/j.issn.1004-0242.2019.02.A014 |
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| 中文关键词: miR-302c 胃肿瘤 侵袭 转移 CREB1 |
| 英文关键词:miR-302c gastric neoplasms migration invasion CREB1 |
| 基金项目:国家重点研发计划资助（2018YFC1313100）；国家自然科学基金资助项目（81430072、81602641、81822031、81572929） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-302c对人胃癌细胞侵袭和迁移能力的影响及机制。［方法］ 实时荧光定量PCR法检测miR-302c在胃癌细胞系和正常胃黏膜上皮细胞中的表达情况。在SGC7901和AGS细胞中分别转染miR-302c mimic和inhibitor后，Tranwell实验检测细胞侵袭和迁移能力变化。实时荧光定量PCR和Western blot法检测转染后环磷酸腺苷反应元件结合蛋白1(CREB1)的变化情况。［结果］与永生化的正常胃黏膜上皮细胞GES-1相比，miR-302c在胃癌细胞系SGC7901、MKN28、N87、MKN45和AGS中表达降低。转染 mimic和inhibitor后，miR-302c在SGC7901和AGS中表达分别明显上调和下调(P＜0.001)。Transwell实验发现，上调miR-302c后，SGC7901细胞的侵袭和迁移能力明显降低，而下调miR-302c后，AGS细胞的侵袭和迁移能力明显增强。进一步研究发现，miR-302c可抑制CREB1的表达。功能挽救实验证明CREB1可部分恢复miR-302c上调对SGC7901细胞侵袭和迁移的抑制作用。［结论］ miR-302c抑制胃癌细胞的侵袭和迁移，其机制可能是通过直接靶向CREB1。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the expression of miR-302c in gastric cancer (GC) cells and its relation to cell migration and invasion. ［Methods］ Real-time quantitative PCR(qRT-PCR) was used to detect miR-302c expression in GCSGC7901，MKN28，MKN45 and AGS cells and immortalized gastric epithelial cell line GES-1. Transwell assay was used to determine the migration and invasion abilities of SGC7901 and AGS cells transfected with miR-302c mimic and inhibitor，respectively. qRT-PCR and Western blot were used to detect the expression of CREB1 after transfection of miR-302c mimic and inhibitor in indicated cells. ［Results］ miR-302c was downregulated in GC cell lines compared with GES-1 cells. Overexpression of miR-302c significantly reduced migration，invasion and CREB1 expression of SGC7901 cells，while silencing of miR-302c significantly increased migration，invasion and CREB1 expression of AGS cells. Overexpression of CREB1 alleviated the decreased migration and invasion induced by miR-302c in SGC7901 cells. ［Conclusion］ miR-302c is downregulated in gastric cancer cell lines and can suppress cancer cell migration and invasion by targeting CREB1. |
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