| 张泽亮,王黎明,任龙飞.AFP通过激活STAT3的磷酸化促进肝癌细胞的增殖[J].中国肿瘤,2018,27(10):796-800. |
| AFP通过激活STAT3的磷酸化促进肝癌细胞的增殖 |
| AFP Promotes Proliferation of Hepatocellular Carcinoma Cells by Activating Phosphorylation of STAT3 |
| 投稿时间：2018-02-05 |
| DOI：10.11735/j.issn.1004-0242.2018.10.A010 |
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| 中文关键词: 肝肿瘤 甲胎蛋白 p-STAT3 增殖 |
| 英文关键词:hepatocellular neoplasms alpha-fetoprotein p-STAT3 proliferation |
| 基金项目:国家自然科学基金面上项目（31270543、31570509)；甘肃卫生行业科研计划管理项目（GWGL2010-22）；甘肃省科技重大专项（1602FKDA001） |
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| 摘要点击次数: 1980 |
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| 中文摘要: |
| 摘 要：［目的］ 研究AFP基因过表达对肝癌细胞系HepG2细胞增殖的影响及其机制。［方法］ 利用慢病毒转染技术对HepG2细胞进行AFP基因过表达，通过Western Blot检测转染后HepG2细胞中AFP表达情况，采用流式细胞技术和CCK8细胞增殖实验检测细胞的增殖情况，Western Blot检测AFP过表达后STAT3、p-STAT3蛋白水平的变化。［结果］ CCK8细胞增殖实验显示，AFP过表达组HepG2细胞较对照组细胞增殖速度快（P<0.05）。流式细胞实验显示，AFP基因过表达组处于G1期和S期的细胞占比分别为49%和21%，而对照组相应的G1期、S期细胞占比分别为61%和12%，差异具有统计学意义（P<0.05）。AFP基因过表达后，HepG2细胞中p-STAT3表达水平升高，表明存在STAT3蛋白的激活。［结论］ AFP可促进HepG2肝癌细胞的增殖，其机制涉及STAT3蛋白的激活。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of AFP on the proliferation of hepatocellular carcinoma cells(HCC). ［Methods］ Lentivirus-mediated recombination AFP was transfected into HCC HepG2 cells. Western Blot was used to detect the efficiency of AFP transfection. The cell proliferation and cell cycle were detected by CCK8 assay and flow cytometry，respectively. The protein level of AFP，STAT3 and p-STAT3 were detected by Western blot. ［Results］ The CCK8 assay indicated that overexpression of AFP significantly enhanced the proliferation of HepG2 cells comparing to the negative control(P<0.05) . G1 and S phase of HepG2 cells in AFP over-expressed HepG2 cells accounted for 49% and 21%，while those in the negative control group accounted for 61% and 12%，respectively(P<0.05). The expression of p-STAT3 was increased in APF over-expressed HepG2 cells. ［Conclusion］ AFP can promote proliferation of HepG2 cells by activating phosphorylation of STAT3. |
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