| 王 磊,许小敏,张艳辉.黄芪多糖对X线辐射所致骨髓间充质干细胞细胞核、染色体及DNA损伤的影响[J].中国肿瘤,2018,27(9):708-714. |
| 黄芪多糖对X线辐射所致骨髓间充质干细胞细胞核、染色体及DNA损伤的影响 |
| Effects of Astragalus Polysaccharide on X-ray Radiation-induced Nucleus，Chromosome and DNA Damage in Bone Mesenchymal Stem Cells |
| 投稿时间：2017-12-27 |
| DOI：10.11735/j.issn.1004-0242.2018.09.A013 |
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| 中文关键词: 黄芪多糖 辐射 细胞核 染色体 DNA |
| 英文关键词:astragalus polysaccharide radiation nucleus chromosome DNA |
| 基金项目:国家自然科学基金项目(81473457) |
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| 摘要点击次数: 1931 |
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| 中文摘要: |
| 摘 要：［目的］ 探索黄芪多糖(astragalus polysaccharide，APS)对X线辐射所致骨髓间充质干细胞(bone mesenchymal stem cell，BMSC)细胞核、染色体及DNA损伤的影响。［方法］ 采用CCK-8法检测不同浓度的APS对2Gy X线辐射后BMSC增殖能力的影响，以筛选出APS的最佳作用浓度。将BMSC分为空白（CTRL）组、黄芪多糖（APS）组、辐射（IR）组、辐射+黄芪多糖（IR+APS）组；APS组、IR+APS组在第1次辐射前48h以最佳作用浓度的APS干预1次，以后每次辐射后均换上含APS的新鲜培养液，CTRL组、IR组则换上不含APS的新鲜培养液，共进行5次X线辐射，每次2Gy，每48h辐射1次；末次辐射后，采用胞质分裂阻断法检测各组细胞微核率，染色体核型分析各组细胞染色体畸变率，激光共聚焦显微镜检测各组细胞53BP1 foci焦点簇数目。［结果］ 与CTRL组比较，辐射后BMSC增殖能力明显受到抑制，差异具有统计学意义(P<0.05)；与IR组比较，当APS为50μg/ml时对辐射后BMSC增殖的作用最强，故选为最佳作用浓度。在细胞核水平上，与CTRL组比较，IR组微核率显著升高(P<0.05)；与IR组比较，IR+APS组微核率降低(P<0.05)。在染色体水平上，与CTRL组比较，IR组染色体畸变率明显升高(P<0.05)；与IR组比较，IR+APS组染色体畸变率降低(P<0.05)。在DNA水平上，与CTRL组比较，IR组53BP1 foci焦点簇数目显著升高(P<0.05)；与IR组比较，IR+APS组53BP1 foci焦点簇数目降低(P<0.05)。［结论］ 黄芪多糖具有减轻X线辐射后BMSC细胞核、染色体、DNA损伤的作用。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effects of astragalus polysaccharide(APS) on the nuclear，chromosome and DNA damage of bone mesenchymal stem cells(BMSC) induced by X-ray radiation.［ Methods］ 50μg/ml was determined as the optimum concentration of APS by pretests. Bone mesenchymal stem cells(BMSC) were divided into CTRL(control) group，APS group，IR(irradiation) group and IR+APS group. BMSCs in IR and IR+APS groups were irradiated for 5 times of 2Gy X-ray radiation every 48h，and BMSCs were treated with different concentrations of APS(0μg/ml，12.5μg/ml，25μg/ml，50μg/ml and 100μg/ml). After the last radiation，the proliferation of BMSCs was determined by CCK-8 assay，the cell micronucleus rate was detected by cytoplasmic blocking，the chromosome aberration rate was detected by chromosome karyotype analysis，and the numbers of 53BP1 foci focal clusters on DNA was detected by laser confocal microscope. ［Results］ Compared with the CTRL group，the proliferation ability of BMSC after irradiation was significantly inhibited(P<0.05). Compared with the IR group，the proliferation of BMSC in APS group of 50μg/ml was the strongest. At the cell nuclear level，the micronucleus rate of IR group was significantly higher than CTRL group(P<0.05)，and compared with IR group，the micronucleus rate of IR+APS group was decreased(P<0.05). At chromosome level，the chromosome aberration rate of IR group was significantly increased compared with CTRL group(P<0.05)，and compared with IR group，the chromosome aberration rate of IR+APS group was declined(P<0.05). At DNA level，the number of 53BP1 foci focal clusters on DNA in IR group was significantly increased than that in CTRL group(P<0.05)，and compared with IR group，the number of 53BP1 foci focal clusters on DNA in IR+APS group was reduced(P<0.05). ［Conclusion］ APS can attenuate the damage of nucleus，chromosome and DNA in BMSC induced by X-ray radiation. |
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