| 谢智钦,李弘夏,唐才喜.辣椒素通过氧化应激诱导HepG2肝癌细胞凋亡的研究[J].中国肿瘤,2018,27(6):470-475. |
| 辣椒素通过氧化应激诱导HepG2肝癌细胞凋亡的研究 |
| Capsaicin Induces Apoptosis in HepG2 Cells Through ROS Activation |
| 投稿时间：2017-06-27 |
| DOI：10.11735/j.issn.1004-0242.2018.06.A013 |
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| 中文关键词: 辣椒素 肝肿瘤 氧化应激 凋亡 |
| 英文关键词:capsaicin hepatocellular neoplasms reactive oxygen species apoptosis |
| 基金项目:湖南省自然科学基金（2017JJ4070）；湖南省科技计划项目(2016SK4006) |
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| 中文摘要: |
| 摘 要：［目的］ 观察辣椒素对HepG2细胞增殖和凋亡的影响及其分子机制。［方法］采用CCK8法观察辣椒素对HepG2细胞增殖的影响，流式细胞术和JC-1探针检测辣椒素对HepG2细胞凋亡率和线粒体膜电位的影响，采用DCFH-DA探针联合高内涵分析仪检测氧化应激表达水平的变化。［结果］ 辣椒素可剂量依赖性抑制HepG2细胞增殖、诱导细胞凋亡。200μmol/L辣椒素作用24h可引起HepG2 细胞明显坏死、脱落和漂浮。相比对照组，200μmol/L辣椒素明显降低HepG2细胞线粒体膜电位红绿荧光表达量比值（8.39±0.12 vs 4.23±0.04）。200μmol/L辣椒素可引起HepG2细胞内氧化应激水平上调1.78倍。使用还原剂维生素C抗氧化可恢复细胞活力，减轻凋亡。［结论］ 辣椒素剂量依赖性诱导人肝癌HepG2细胞凋亡，其机制与氧化应激水平过激活相关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effects of capsaicin on the proliferation and apoptosis of HepG2 cells. ［Methods］ Human liver cancer HepG2 cells were treated with 200μmol/L capsaicin for 12h or 24h. The cell proliferation was assessed with cell counting kit-8(CCK-8)；cell apoptosis was detected with flow cytometry；mitochondrial membrane potential changes were determined by JC-1 probe staining；the oxidative stress was determined by DCFH-DA probe in combination with high-throughput screening(HTS) applications. ［Results］ Capsaicin showed anti-proliferative and pro-apoptotic effects on HepG2 cells in a dose-dependent manner(P<0.01). Compared with untreated cells，the mitochondrial membrane potential in HepG2 cells was decreased after treatment of 200μmol/L capsaicin for 24h（8.39±0.12 vs 4.23±0.04，P<0.01）.The level of reactive oxygen species(ROS) in HepG2 cells was increased by 1.78 times after capsaicin treatment. The capsaicin-induced cell damage and apoptosis were alleviated by pretreatment of antioxidant Vitamin C. ［Conclusion］ Capsaicin induces apoptosis in human liver cancer HepG2 cells through the activation of reactive oxygen species. |
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