| 刘 磊,沈素朋,邝 钢.MiR-4659b和长链非编码RNA XLOC_008370在食管鳞癌中的表达及临床意义[J].中国肿瘤,2017,26(5):400-404. |
| MiR-4659b和长链非编码RNA XLOC_008370在食管鳞癌中的表达及临床意义 |
| Expressions of MiR-4659b and LncRNA XLOC_008370 in Esophageal Squamous Cell Carcinoma and Their Clinicopathological Significance |
| 投稿时间：2016-11-03 |
| DOI：10.11735/j.issn.1004-0242.2017.05.A015 |
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| 中文关键词: 食管癌 微小RNA 长链非编码RNA 淋巴结转移 |
| 英文关键词:esophageal cancer microRNA long non-coding RNA lymph node metastasis |
| 基金项目:国家自然科学基金资助项目（81572441）；河北省自然科学基金（H2015206420） |
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| 中文摘要: |
| 摘 要：［目的］ 检测食管鳞状细胞癌（esophageal squamous cell carcinoma， ESCC）组织中微小RNA 4659b（microRNA-4659b，miR-4659b）和长链非编码RNA XLOC_008370（long non-coding RNA XLOC_008370，lncRNA XLOC_008370）的表达，探讨miR-4659b和XLOC_008370在ESCC发生及发展中的作用机制。［方法］ 应用实时荧光定量PCR（real-time quantitative polymerase chain reaction，qRT-PCR）的方法检测miR-4659b和XLOC_008370在50例ESCC组织中的表达情况及相关性。［结果］ ESCC组织中miR-4659b的相对表达量显著高于相应癌旁组织［(0.034±0.013) vs (0.005±0.002)，t=15.205， P<0.05］。miR-4659b的相对表达量与ESCC患者淋巴结转移有关 ［（0.039±0.011）vs（0.023±0.009），t=5.708，P<0.05］。XLOC_008370在ESCC中的相对表达量显著低于相应癌旁组织［（0.0019±0.0012）vs（0.020±0.0146），t=-8.921，P<0.05］。XLOC_008370的相对表达量与ESCC患者淋巴结转移有关［（0.0016±0.0010）vs（0.023±0.0014），t=-2.040， P<0.05］，并且XLOC_008370与miR-4659b的相对表达量呈负相关（r=-0.829，P<0.05）。［结论］ miR-4659b在ESCC中表达上调而XLOC_008370表达降低，且两者之间有相关性，提示miR-4659b与XLOC_008370可能存在一定的调控作用，这一机制可能在ESCC发生发展中起重要作用。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the expressions of microRNA-4659b and long non-coding RNA (lncRNA) XLOC_008370 in esophageal squamous cell carcinoma (ESCC) and their relations to clinicopathological features. ［Methods］ Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expressions of microRNA-4659b and lncRNA XLOC_008370 in 50 ESCC patients，and their clinicopathological significance was studied. ［Results］ The expression of miR-4659b in ESCC tumor tissues was significantly higher than that in cancer adjacent tissues ［(0.034±0.013) vs (0.005±0.002)，t=15.205，P<0.05］ and the expression was higher in ESCC with lymph node metastasis than that in non-metastatic ESCC［(0.039±0.011) vs (0.023±0.009)，t=5.708，P<0.05］. The expression of XLOC_008370 in ESCC tumor tissues was significantly lower than that in cancer adjacent tissues ［(0.0016±0.0010) vs (0.023±0.0014)，t=-2.04，P<0.05］ and the expression was lower in ESCC with lymph node metastasis than that in non-metastatic ESCC ［(0.039±0.011) vs (0.023±0.009)，t=5.708，P<0.05］. The relative expression of XLOC_008370 was negatively correlated with miR-4659b（r=-0.829，P<0.05）. ［Conclusion］ The expression of miR-4659b is up-regulated while XLOC_008370 is down-regulated in ESCC，and they are negatively correlated with each other. Results suggest that MiR-4659b may target to regulate XLOC_008370，and it might be involved in the development of ESCC. |
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