| 丁 凯,尹 丽,顾佳佳.ZNF488对电离辐射诱发的鼻咽癌细胞CNE1侵袭迁移能力的影响[J].中国肿瘤,2017,26(5):395-399. |
| ZNF488对电离辐射诱发的鼻咽癌细胞CNE1侵袭迁移能力的影响 |
| Effect of ZNF488 on Ionizing Irradiation-induced Migration and Invasiveness of Nasopharyngeal Cancer CNE1 Cells |
| 投稿时间：2017-02-26 |
| DOI：10.11735/j.issn.1004-0242.2017.05.A014 |
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| 中文关键词: ZNF488 侵袭 迁移 电离辐射 鼻咽癌 上皮间质转化 |
| 英文关键词:ZNF488 migration invasion ionizing radiation nasopharyngeal cancer epithelial mesenchymal transition |
| 基金项目:江苏省科技厅临床医学重点专项（BL2014091） |
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| 中文摘要: |
| 摘 要：［目的］ 研究ZNF488 siRNA对电离辐射诱发的鼻咽癌CNE1侵袭迁移能力的影响。 ［方法］采用qRT-PCR和Western blot检测ZNF488 siRNA转染效率。采用划痕实验和Transwell侵袭实验检测电离辐射对鼻咽癌CNE1细胞侵袭迁移能力的影响。采用划痕实验和Transwell侵袭实验检测沉默ZNF488后，电离辐射诱发的CNE1细胞侵袭迁移能力是否改变。［结果］ 在mRNA和蛋白水平，实验组（siZNF488组）表达量均明显低于对照组（siRNA-Ctrl组），其中实验组ZNF488 mRNA水平为对照组的（0.54±0.12）倍（P=0.023）。划痕实验证明电离辐射明显增强鼻咽癌CNE1细胞迁移能力；Transwell侵袭实验检测到0Gy组侵袭细胞数为302.67±18.77，4Gy组侵袭细胞数为371.67±15.63，4Gy组侵袭细胞数为0Gy组（1.23±0.03）倍（P=0.006）。沉默ZNF488后，电离辐射诱发的CNE1细胞侵袭迁移能力明显减弱，这一功能的实现与上皮间质转化（EMT）进程的逆转息息相关。［结论］ ZNF488 siRNA 通过逆转EMT进程抑制鼻咽癌细胞CNE1电离辐射诱发的侵袭迁移能力。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of ZNF488 on ionizing irradiation (IR)-induced migration and invasion in nasopharyngeal cancer CNE1 cells. ［Methods］ ZNF488 siRNA was transfected into CNE1 cells，the expression of ZNF488 mRNA and protein was detected by qRT-PCR and Western blot after transfection，respectively. The transfected and non-transfected CNE1 cells were exposed to 200cGy/min X-ray. The invasion and migration of CNE1 cells were analyzed by Transwell invasion assay and scratch wound healing. ［Results］ The mRNA expression level of ZNF488 in transfected CNE1 cells was (0.54±0.12) times as in control group (P =0.023). Transwell test showed that the number of invasion cells in 4Gy IR group(371.67±15.63) was (1.23±0.03) times higher than that of 0Gy group(302.67±18.77). The expression of ZEB1 decreased and expression of E-cadherin increased in CNE1 cells after transfected with siZNF488，while the expression of ZEB1 increased and E-cadherin decreased after 4Gy IR. ［Conclusion］ Silencing ZNF488 could be a new target for inhibiting metastasis caused by IR，which may be associated with regulation of IR-induced epithelial mesenchymal transition (EMT). |
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