| 张征峥,马翠卿,邓郁青.miR-155参与缺氧诱导的乳腺癌化疗耐药[J].中国肿瘤,2017,26(2):135-139. |
| miR-155参与缺氧诱导的乳腺癌化疗耐药 |
| MiR-155 Participates in the Breast Cancer Chemoresistance Induced by Hypoxia |
| 投稿时间：2016-05-05 |
| DOI：10.11735/j.issn.1004-0242.2017.02.A012 |
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| 中文关键词: miR-155 缺氧 乳腺癌 化疗耐药 BCRP |
| 英文关键词:miR-155 hypoxia breast cancer chemoresistance BCRP |
| 基金项目:国家自然科学基金面上项目（81071710）；河北省高等学校科学技术研究项目（QN2015011） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-155在缺氧诱导的乳腺癌化疗耐药中的作用。［方法］ 以0、50、100、150、200μmol/L不同浓度CoCl2处理4T1细胞，建立缺氧模型。实时定量PCR检测缺氧条件下miR-155的表达与耐药基因BCRP的表达。通过慢病毒载体转染使miR-155过表达，通过caspase 3/9分光光度法探讨miR-155在缺氧环境下乳腺癌化疗耐药中的作用。［结果］ 随缺氧程度的增加，miR-155及BCRP基因表达增加，过表达miR-155可抑制阿霉素诱导的4T1细胞的凋亡。［结论］ 随着乳腺癌缺氧程度加重，miR-155表达逐渐增加，miR-155表达与乳腺癌化疗耐药相关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of miR-155 on the drug resistance of breast cancer. ［Methods］ 4T1 cells were treated by 0，50，100，150，200μmol/L CoCl2. Real time quantitative PCR was conducted to detect the expression of miR-155 and BCRP，a drug resistance gene，under hypoxic condition. To further explore the role of miR-155 in chemoresistance，a lentiviral vector carrying miR-155 precursor was transfected into 4T1 cells. Then the caspase 3/9 activity was conducted by spectrophotometric method. ［Results］ With the increase of hypoxia，the expression of miR-155 and BCRP gene increased. Over expression of miR-155 repress apoptosis induced by doxorubicin.［Conclusion］ With the increasing of hypoxia，the expression of miR-155 increases. MiR-155 expression is associated with chemotherapy resistance in breast cancer. |
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